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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Borrelia recurrentis (strain A1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathway: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophileUniRule annotation
Binding sitei91 – 911SubstrateUniRule annotation
Metal bindingi305 – 3051ZincUniRule annotation
Metal bindingi307 – 3071ZincUniRule annotation
Metal bindingi310 – 3101ZincUniRule annotation
Metal bindingi336 – 3361ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBREC412418:GJIA-820-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:BRE_820
OrganismiBorrelia recurrentis (strain A1)
Taxonomic identifieri412418 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorrelia
ProteomesiUP000000612 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_1000097533Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

B5RQE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNIIKNDKN SNARLGILEL PHGNVATPCF MPVGTLGVMK ALKHDVLEKL
60 70 80 90 100
GCDLMLANTY HLYLRPGIDV IKKYGNLHNF TTWNKNFLTD SGGFQVFSLS
110 120 130 140 150
NFRKIEDEGV DFKSHIDGSR HYFTPESVFS MQETFESDII MALDICSPYG
160 170 180 190 200
IDYDEASLYT NITTSWARRT LCAYKNRKEG YEGLLFLITQ GNFFKDLRKR
210 220 230 240 250
STELILELNS PGIAIGGISV GEPRDRYLEI LEYNSSLIPK DKPKYVMGIG
260 270 280 290 300
TPHYILDAIY NGIDIFDCVN PTRIARHGSL LTDNGILRIN RAEFCFDTCS
310 320 330 340 350
VERECSCTLC TRYSRGYLRH LFKSEEALGV MLASEHNIHY MFRLINKTKN
360 370
AIMNDNFVKF RKLYLDKYDE GNLNE
Length:375
Mass (Da):42,950
Last modified:November 4, 2008 - v1
Checksum:iD7EE88EE2F85C5B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH95031.1.
RefSeqiWP_012539183.1. NC_011244.1.
YP_002223252.1. NC_011244.1.

Genome annotation databases

EnsemblBacteriaiACH95031; ACH95031; BRE_820.
KEGGibre:BRE_820.
PATRICi20570813. VBIBorRec40566_0825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH95031.1.
RefSeqiWP_012539183.1. NC_011244.1.
YP_002223252.1. NC_011244.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH95031; ACH95031; BRE_820.
KEGGibre:BRE_820.
PATRICi20570813. VBIBorRec40566_0825.

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciBREC412418:GJIA-820-MONOMER.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1.

Entry informationi

Entry nameiTGT_BORRA
AccessioniPrimary (citable) accession number: B5RQE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 4, 2008
Last modified: June 24, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.