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B5RQE7

- TGT_BORRA

UniProt

B5RQE7 - TGT_BORRA

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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Borrelia recurrentis (strain A1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophileUniRule annotation
Binding sitei91 – 911SubstrateUniRule annotation
Metal bindingi305 – 3051ZincUniRule annotation
Metal bindingi307 – 3071ZincUniRule annotation
Metal bindingi310 – 3101ZincUniRule annotation
Metal bindingi336 – 3361ZincUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBREC412418:GJIA-820-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:BRE_820
OrganismiBorrelia recurrentis (strain A1)
Taxonomic identifieri412418 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000000612: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_1000097533Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi412418.BRE_820.

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiIVQGAFF.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

B5RQE7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNIIKNDKN SNARLGILEL PHGNVATPCF MPVGTLGVMK ALKHDVLEKL
60 70 80 90 100
GCDLMLANTY HLYLRPGIDV IKKYGNLHNF TTWNKNFLTD SGGFQVFSLS
110 120 130 140 150
NFRKIEDEGV DFKSHIDGSR HYFTPESVFS MQETFESDII MALDICSPYG
160 170 180 190 200
IDYDEASLYT NITTSWARRT LCAYKNRKEG YEGLLFLITQ GNFFKDLRKR
210 220 230 240 250
STELILELNS PGIAIGGISV GEPRDRYLEI LEYNSSLIPK DKPKYVMGIG
260 270 280 290 300
TPHYILDAIY NGIDIFDCVN PTRIARHGSL LTDNGILRIN RAEFCFDTCS
310 320 330 340 350
VERECSCTLC TRYSRGYLRH LFKSEEALGV MLASEHNIHY MFRLINKTKN
360 370
AIMNDNFVKF RKLYLDKYDE GNLNE
Length:375
Mass (Da):42,950
Last modified:November 4, 2008 - v1
Checksum:iD7EE88EE2F85C5B8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000993 Genomic DNA. Translation: ACH95031.1.
RefSeqiYP_002223252.1. NC_011244.1.

Genome annotation databases

EnsemblBacteriaiACH95031; ACH95031; BRE_820.
GeneIDi6919445.
KEGGibre:BRE_820.
PATRICi20570813. VBIBorRec40566_0825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000993 Genomic DNA. Translation: ACH95031.1 .
RefSeqi YP_002223252.1. NC_011244.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 412418.BRE_820.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH95031 ; ACH95031 ; BRE_820 .
GeneIDi 6919445.
KEGGi bre:BRE_820.
PATRICi 20570813. VBIBorRec40566_0825.

Phylogenomic databases

eggNOGi COG0343.
HOGENOMi HOG000223473.
KOi K00773.
OMAi IVQGAFF.
OrthoDBi EOG6SNDVG.

Enzyme and pathway databases

UniPathwayi UPA00392 .
BioCyci BREC412418:GJIA-820-MONOMER.

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1.

Entry informationi

Entry nameiTGT_BORRA
AccessioniPrimary (citable) accession number: B5RQE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 4, 2008
Last modified: October 1, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3