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B5RQE7

- TGT_BORRA

UniProt

B5RQE7 - TGT_BORRA

Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Borrelia recurrentis (strain A1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (04 Nov 2008)
      Previous versions | rss
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    Functioni

    Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

    Catalytic activityi

    Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
    Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901NucleophileUniRule annotation
    Binding sitei91 – 911SubstrateUniRule annotation
    Metal bindingi305 – 3051ZincUniRule annotation
    Metal bindingi307 – 3071ZincUniRule annotation
    Metal bindingi310 – 3101ZincUniRule annotation
    Metal bindingi336 – 3361ZincUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Queuosine biosynthesis, tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBREC412418:GJIA-820-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
    Alternative name(s):
    Guanine insertion enzymeUniRule annotation
    tRNA-guanine transglycosylaseUniRule annotation
    Gene namesi
    Name:tgtUniRule annotation
    Ordered Locus Names:BRE_820
    OrganismiBorrelia recurrentis (strain A1)
    Taxonomic identifieri412418 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
    ProteomesiUP000000612: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_1000097533Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi412418.BRE_820.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0343.
    HOGENOMiHOG000223473.
    KOiK00773.
    OMAiIVQGAFF.
    OrthoDBiEOG6SNDVG.

    Family and domain databases

    Gene3Di3.20.20.105. 1 hit.
    HAMAPiMF_00168. Q_tRNA_Tgt.
    InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view]
    PANTHERiPTHR11962. PTHR11962. 1 hit.
    PfamiPF01702. TGT. 1 hit.
    [Graphical view]
    SUPFAMiSSF51713. SSF51713. 1 hit.
    TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B5RQE7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNIIKNDKN SNARLGILEL PHGNVATPCF MPVGTLGVMK ALKHDVLEKL    50
    GCDLMLANTY HLYLRPGIDV IKKYGNLHNF TTWNKNFLTD SGGFQVFSLS 100
    NFRKIEDEGV DFKSHIDGSR HYFTPESVFS MQETFESDII MALDICSPYG 150
    IDYDEASLYT NITTSWARRT LCAYKNRKEG YEGLLFLITQ GNFFKDLRKR 200
    STELILELNS PGIAIGGISV GEPRDRYLEI LEYNSSLIPK DKPKYVMGIG 250
    TPHYILDAIY NGIDIFDCVN PTRIARHGSL LTDNGILRIN RAEFCFDTCS 300
    VERECSCTLC TRYSRGYLRH LFKSEEALGV MLASEHNIHY MFRLINKTKN 350
    AIMNDNFVKF RKLYLDKYDE GNLNE 375
    Length:375
    Mass (Da):42,950
    Last modified:November 4, 2008 - v1
    Checksum:iD7EE88EE2F85C5B8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000993 Genomic DNA. Translation: ACH95031.1.
    RefSeqiYP_002223252.1. NC_011244.1.

    Genome annotation databases

    EnsemblBacteriaiACH95031; ACH95031; BRE_820.
    GeneIDi6919445.
    KEGGibre:BRE_820.
    PATRICi20570813. VBIBorRec40566_0825.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000993 Genomic DNA. Translation: ACH95031.1 .
    RefSeqi YP_002223252.1. NC_011244.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 412418.BRE_820.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACH95031 ; ACH95031 ; BRE_820 .
    GeneIDi 6919445.
    KEGGi bre:BRE_820.
    PATRICi 20570813. VBIBorRec40566_0825.

    Phylogenomic databases

    eggNOGi COG0343.
    HOGENOMi HOG000223473.
    KOi K00773.
    OMAi IVQGAFF.
    OrthoDBi EOG6SNDVG.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci BREC412418:GJIA-820-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.105. 1 hit.
    HAMAPi MF_00168. Q_tRNA_Tgt.
    InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view ]
    PANTHERi PTHR11962. PTHR11962. 1 hit.
    Pfami PF01702. TGT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51713. SSF51713. 1 hit.
    TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
      Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
      PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: A1.

    Entry informationi

    Entry nameiTGT_BORRA
    AccessioniPrimary (citable) accession number: B5RQE7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 4, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3