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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfk

Organism
Borrelia recurrentis (strain A1)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881ATP; via amide nitrogenUniRule annotation
Metal bindingi180 – 1801Magnesium; catalyticUniRule annotation
Sitei181 – 1811Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei210 – 2101Proton acceptorUniRule annotation
Binding sitei315 – 3151SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi154 – 1552ATPUniRule annotation
Nucleotide bindingi179 – 1824ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: InterPro
  2. ATP binding Source: InterPro
  3. diphosphate-fructose-6-phosphate 1-phosphotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBREC412418:GJIA-733-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkImported
Synonyms:pfkAUniRule annotation
Ordered Locus Names:BRE_733Imported
OrganismiBorrelia recurrentis (strain A1)Imported
Taxonomic identifieri412418 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000000612 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi412418.BRE_733.

Structurei

3D structure databases

ProteinModelPortaliB5RQ66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni208 – 2103Substrate bindingUniRule annotation
Regioni253 – 2553Substrate bindingUniRule annotation
Regioni368 – 3714Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
KOiK00850.
OMAiFGFQTAF.
OrthoDBiEOG6T7N42.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

B5RQ66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRLKNQNLG FNIENLGECK YDNPLINFYA NESHVHFTSE ESKIRFNVYK
60 70 80 90 100
NEESWNQYED VFLEKAGPRR KIYFVPKHVK AAITTCGGLC PGFNDVIRSI
110 120 130 140 150
VRTLWNVYGV YNIYGVKFGY KGLLSESNLS FVDLNPDVVN DINQLGGTIL
160 170 180 190 200
GSSRGGIRPV EIVDTLERMG INMLFNIGGD GTQKGSILIA EEIERRNLKI
210 220 230 240 250
AVIGIPKTVD NDFMFVQKSF GFETAVEQSV AAVAGAHFEA NSAYNGIGLV
260 270 280 290 300
KVMGRDSGFI AAYTALSSND VNFCLIPELD FDIEGPNGLL AHLERRLLQK
310 320 330 340 350
ESMDEIPHAV ILIAEGAGQK YFDPGIRKKD DSGNLLHEDI GLYIKDKINE
360 370 380 390 400
YFKSKDIPVN LKYIDPSYII RSSPANASDS LYCARLGSNA VHAAMAGKTK
410 420 430 440
LLISLWSTKF VHVPIKMAVI ARNKVNVNGS FWRDVLASTG QPFSMKN
Length:447
Mass (Da):49,721
Last modified:November 4, 2008 - v1
Checksum:i62E0EB0F7726B280
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH94950.1.
RefSeqiYP_002223171.1. NC_011244.1.

Genome annotation databases

EnsemblBacteriaiACH94950; ACH94950; BRE_733.
KEGGibre:BRE_733.
PATRICi20570631. VBIBorRec40566_0738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000993 Genomic DNA. Translation: ACH94950.1.
RefSeqiYP_002223171.1. NC_011244.1.

3D structure databases

ProteinModelPortaliB5RQ66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi412418.BRE_733.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH94950; ACH94950; BRE_733.
KEGGibre:BRE_733.
PATRICi20570631. VBIBorRec40566_0738.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
KOiK00850.
OMAiFGFQTAF.
OrthoDBiEOG6T7N42.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciBREC412418:GJIA-733-MONOMER.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1Imported.

Entry informationi

Entry nameiB5RQ66_BORRA
AccessioniPrimary (citable) accession number: B5RQ66
Entry historyi
Integrated into UniProtKB/TrEMBL: November 4, 2008
Last sequence update: November 4, 2008
Last modified: April 29, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.