Reviewed, UniProtKB/Swiss-Prot B5RQ59 (SYT_BORRA)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BRE_726

Organism

Borrelia recurrentis (strain A1) [Complete proteome] [HAMAP]

Taxonomic identifier

412418 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000098546
Regions
	Region	185 – 478	294	Catalytic HAMAP MF_00184
Sites
	Metal binding	278	1	Zinc; catalytic By similarity
	Metal binding	329	1	Zinc; catalytic By similarity
	Metal binding	455	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5RQ59-1 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: 37A362B57C441008	FASTA	583	68,581
	10 20 30 40 50 60 MGEKLDKDSI LYKKRHSIAH VMAEAVLELF PNTKIAIGPP IKDGFYYDFD FEKHISEDDL 70 80 90 100 110 120 LLIEHKMREI LKTGSPFIRE VITREQALVL FKDEPYKIDL IQNFDVTDEI TIYKSHKFTD 130 140 150 160 170 180 LCRGPHVDNM NKLDPKAFKL TSIAGAYWRG NERNKMLSRI YGTLWNNEKD LKAYLKLQEE 190 200 210 220 230 240 IKKRDHRKLG RELNLFSVHD EIGPGLIFFH PHGARIRALI ENFWREEHFK NGYDILFTPH 250 260 270 280 290 300 IGKSWLWETS GHLDFYKESM FEKIEMDRSD YYVKPMNCPF HIAIYNTDKH SYRDLPFRWA 310 320 330 340 350 360 ELGTVYRYEK IGAIHGTMRV RGFTQDDAHI ICTYEQVNFE VREVLRFAID MWNKFGFTNL 370 380 390 400 410 420 KAYLSTKPEK AVGDDDDWQM AVKVLEKALI DFNIDFDIDE GGGAFYGPKI DLKIIDSLGR 430 440 450 460 470 480 AWQMTTIQFD FNLPVRFKMT YTAEDGKEKR PFMIHRALLG SIERFFGILV EHYGGAFPVW 490 500 510 520 530 540 LAPLQVVIIP VNSIVEEYAL EVLSRFQNEG IRIKFDNYYN MRMNAKIRQY QSKKVPYMFI 550 560 570 580 IGEREVVEGK ISIRTRTNEQ INGLELKEAL EFVKLKISNK EIL

References

[1]

"The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii." Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 4:E1000185-E1000185(2008) [PubMed: 18787695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000993 Genomic DNA. Translation: ACH94943.1.
RefSeq	YP_002223164.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6919268.
GenomeReviews	Gene locus BRE_726 in contig CP000993_GR.
KEGG	bre:BRE_726.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	FCQHFTL.
Family and domain databases
HAMAP	MF_00184_B. Thr_tRNA_synth_B. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BORRA

Accession

Primary (citable) accession number: B5RQ59

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 4, 2008
Last modified:	February 9, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)