B5RQ39 (TRMD_BORRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BRE_704

Organism

Borrelia recurrentis (strain A1) [Complete proteome] [HAMAP]

Taxonomic identifier

412418 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000130139
Regions
	Region	130 – 135	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	110	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5RQ39 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: A6AC1A663E1C8CE3	FASTA	240	27,441
	10 20 30 40 50 60 MKITILSLFP SIITPFFENS IMKKVVDKGI ISYEVISIRD FSKDKHKRCD DVPYGGGAGM 70 80 90 100 110 120 VLKVQPIVDA LEYVNANSKT TIFVSPSGLK YTQKLAYDLS KKDELVIICG RYEGLDQRVI 130 140 150 160 170 180 DLYVDLEISV GDYVLSSGEV AALVIIDSVY RLLDGVINPN SLCEESFSFE CGLLEYPHYT 190 200 210 220 230 240 RPYEFRNLKV PDVLLSGHHE EIKKWRLVKS VEKTKKNRFD LYLKYLEMRG EKNDGFDKKN

References

[1]

"The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii." Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 4:E1000185-E1000185(2008) [PubMed: 18787695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000993 Genomic DNA. Translation: ACH94923.1.
RefSeq	YP_002223144.1. NC_011244.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5RQ39.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000005153; EBBORP00000004728; EBBORG00000005153.
GeneID	6919246.
GenomeReviews	Gene locus BRE_704 in contig CP000993_GR.
KEGG	bre:BRE_704.
PATRIC	20570571. VBIBorRec40566_0710.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007285.
HOGENOM	HBG285805.
OMA	NRYDLYL.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BORRA

Accession

Primary (citable) accession number: B5RQ39

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 4, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program