Reviewed, UniProtKB/Swiss-Prot B5RPT6 (SYM_BORRA)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BRE_592

Organism

Borrelia recurrentis (strain A1) [Complete proteome] [HAMAP]

Taxonomic identifier

412418 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_1000093699
Regions
	Domain	562 – 667	106	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	330 – 334	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity
	Metal binding	158	1	Zinc By similarity
	Binding site	333	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5RPT6-1 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: C8BF1CECCF007780	FASTA	726	84,507
	10 20 30 40 50 60 MQKKNLITAA LPYVNNIPHL GNLVQVLSAD AFARYSRMMG IETLYVCGTD EYGTATETKA 70 80 90 100 110 120 LIEKTTPEEL CNKYHAIHKS IYEWFNIKFD IFGRTTNKYH KETVQDLFLK LDKNGYITEK 130 140 150 160 170 180 ENEQFFCQQD QMFLADRYVT GECPNCGNNT KGDQCENCSN LLVTNELLNP RCIICKNIPI 190 200 210 220 230 240 IKKTKHLYID LPKIKNELEH WIQQIDQNTN WNINAIKITN AFLRDGLKER TITRDLKWGI 250 260 270 280 290 300 PVPKKEYENK VFYVWFDAPI GYISITKEII KDWESWWKNN EDTNLIQFIG KDNILFHTIM 310 320 330 340 350 360 FPSIELGSQE NWTMLNKLAS SEYLNYENLK FSKSAGTGIF GNDVITTEIP SDVWRFYIYY 370 380 390 400 410 420 NRPEKADFQF MWDDFMERIN SELIGNFSNL INRVLTFYKK FFGDKIDKIE LNENFWQIVN 430 440 450 460 470 480 IKYERTINFF KQIELKAALK EILDISRIGN KIFQDKEPWK TKNSTPQTTK ELLLNLIYLI 490 500 510 520 530 540 RDLSILISPF MPHTSDRIRS FFGKSYEISN KFLGTNLGLT TIQSTEVLFT KLEKQLIDSL 550 560 570 580 590 600 KLKYSGRTNM QDEKNKNSIN LFSEQICLKT VKIKTIDRNP DAEKLFILKL DDGTPEGKQI 610 620 630 640 650 660 VSSIADHYTE EELIGKHIII VDNLKPAKFR GIRSEGMLIA TKDENKNFKI IIVEDFKDNP 670 680 690 700 710 720 IPGERVILES DTGKELKSPT KISIDKFLQA QIVAENGELK INGINLILEH SKEKVLSKEI PNGKIY

References

[1]

"The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii." Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 4:E1000185-E1000185(2008) [PubMed: 18787695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000993 Genomic DNA. Translation: ACH94820.1.
RefSeq	YP_002223041.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6919134.
GenomeReviews	Gene locus BRE_592 in contig CP000993_GR.
KEGG	bre:BRE_592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SAYQPEQ.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BORRA

Accession

Primary (citable) accession number: B5RPT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 4, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)