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B5RN32

- SYI_BORDL

UniProt

B5RN32 - SYI_BORDL

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BDU_846
Organism
Borrelia duttonii (strain Ly)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBDUT412419:GJ78-846-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BDU_846
OrganismiBorrelia duttonii (strain Ly)
Taxonomic identifieri412419 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000000611: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216249Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi412419.BDU_846.

Structurei

3D structure databases

ProteinModelPortaliB5RN32.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

B5RN32-1 [UniParc]FASTAAdd to Basket

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MFKKVENKVH FPQLEEKILQ FWNDNKIFEK SMKQREGCEE FTFYDGPPFA     50
TGLPHFGHFV PNTIKDIIPR YQTMKGKHVK RYFGWDTHGL PVEYEVEKSL 100
KLSGRYEIEQ YGIDKFNEEC RNIVLRYTKE WKKIITRLGR WVDFENNYKT 150
MDLTFMESVW WVFKTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
EIHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGK DIDYSKVLDQ 250
EKNEIYIIGT KRLNHYYQDE NKYVIIEQFK GEHLKGIEYE PLFDYFVNQR 300
NKGAFKIHTA EYVTTDDGTG IVHIAPFGEE DYQILKKNTQ TDMITPIDAE 350
CKFTSEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENYLH RYPFCYRTNS 400
PLIYRPISSW FVNIEKIKEK LIRSNEQINW IPEHLKKGRF GKWLENARDW 450
AISRNRFWGN PIPIWKCSKT GNKICIGSRE ELEKLSGQKI IDLHKDKIDK 500
ITWPSKYGGT YVRTSEVLDC WFESGSMPYA SKHYPFKDKD KFQNIFPADF 550
IAEGLDQTRG WFYTLTILGT ALFEKTAFKN VIVNGLVLSS DGKKMSKSLK 600
NYTDPIQIIN TFGADALRLY LIMSPVIKAD DLKYSDDGVK DVLKNIIIPI 650
WNAYSFFITY AIIDKFTPNN HVNLYKTNIL DKWIISEIES LKQILNEEID 700
KYNLTKSIDV LLTFIDKLNN WYIRRSRRRF WKSENDNDKT DAYETLYYTL 750
KNLMLMLAPF IPFLTEEIYQ NLKTKNEKES IHLNDYPQSI KELINIELEE 800
KMNFTRKVIT IARALRASHN IKIRKPIKTI YIITKNHKEQ NTLREMTEII 850
LEEINAKEIK IKSNEEELVT YKAKANFKEL GSKLGTNMKS VALAITKLSN 900
EDILEIINGN KHTITINNNT YDITLKDIIL ERHERKNLKV INEDSITIGL 950
DTLITEELYL EGLSRELIRK VQNLRKESNF NVTDRIILYT NNDEILTKII 1000
NNFENYIKTE TLAITIEINN KKALKTLELD EEISVNIGIE KCLN 1044
Length:1,044
Mass (Da):122,965
Last modified:November 4, 2008 - v1
Checksum:i96679F4DE629A8E5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000976 Genomic DNA. Translation: ACH93768.1.
RefSeqiWP_012538573.1. NC_011229.1.
YP_002222474.1. NC_011229.1.

Genome annotation databases

EnsemblBacteriaiACH93768; ACH93768; BDU_846.
GeneIDi6918072.
KEGGibdu:BDU_846.
PATRICi20563818. VBIBorDut9941_0853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000976 Genomic DNA. Translation: ACH93768.1 .
RefSeqi WP_012538573.1. NC_011229.1.
YP_002222474.1. NC_011229.1.

3D structure databases

ProteinModelPortali B5RN32.
ModBasei Search...

Protein-protein interaction databases

STRINGi 412419.BDU_846.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH93768 ; ACH93768 ; BDU_846 .
GeneIDi 6918072.
KEGGi bdu:BDU_846.
PATRICi 20563818. VBIBorDut9941_0853.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BDUT412419:GJ78-846-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ly.

Entry informationi

Entry nameiSYI_BORDL
AccessioniPrimary (citable) accession number: B5RN32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 4, 2008
Last modified: September 3, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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