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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Borrelia duttonii (strain Ly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi121 – 1211ZincUniRule annotation
Metal bindingi123 – 1231ZincUniRule annotation
Metal bindingi148 – 1481ZincUniRule annotation
Metal bindingi150 – 1501ZincUniRule annotation
Binding sitei269 – 2691ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBDUT412419:GJ78-367-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:BDU_367
OrganismiBorrelia duttonii (strain Ly)
Taxonomic identifieri412419 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000000611: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Glutamate--tRNA ligasePRO_0000367619Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi412419.BDU_367.

Structurei

3D structure databases

ProteinModelPortaliB5RLR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi24 – 3411"HIGH" regionAdd
BLAST
Motifi266 – 2705"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B5RLR1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIFQKRTFFI KRGCVLNIRV RYAPSPTGLQ HIGGIRTALF NYFFAKSFNG
60 70 80 90 100
KFLLRIEDTD QTRYYKEAEE DLYQSLAWLG IDFDEGPTCG GSYSPYIQSQ
110 120 130 140 150
RTEIYRKYAK ELIESGNAYY CYCSPDRLER IRKIQTINKM APGYDRHCRH
160 170 180 190 200
LNKDEIKDAL SLGISPVIRF KIPFDGETSF NDILLGKITW ANKDISPDPV
210 220 230 240 250
ILKSDGFPTY HLANVVDDHL MEISHVLRAQ EWISSGPLHV LLYNAFRWNP
260 270 280 290 300
PIYCHLPMVM GSDGQKLSKR HGATALKQFI DDGYLPEAII NYVTLLGWSY
310 320 330 340 350
DGKSEFFTKN ELQKLFSIDK ISKSPAVFDY NKLDFFNSHY IRTKEDHELA
360 370 380 390 400
ELLLPFLQKA GYIKKDSNSC DKEKLLLLVP LIKPRIRKLG DAVGMLRFFY
410 420 430 440 450
TNISTWNLNE FLGKKKTVRD IYLLLEKIKP VLEGFETRIL SENEQIFYNF
460 470 480 490 500
AKENDLKIGE VLLPIRIAVL GSKVSPPLFD SLQLLGKVTV FDRINKAQDF

LKKYEL
Length:506
Mass (Da):58,556
Last modified:November 4, 2008 - v1
Checksum:i4BAB79F89CB51DA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000976 Genomic DNA. Translation: ACH93319.1.
RefSeqiWP_012538130.1. NC_011229.1.
YP_002222025.1. NC_011229.1.

Genome annotation databases

EnsemblBacteriaiACH93319; ACH93319; BDU_367.
GeneIDi6918451.
KEGGibdu:BDU_367.
PATRICi20562842. VBIBorDut9941_0394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000976 Genomic DNA. Translation: ACH93319.1.
RefSeqiWP_012538130.1. NC_011229.1.
YP_002222025.1. NC_011229.1.

3D structure databases

ProteinModelPortaliB5RLR1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi412419.BDU_367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH93319; ACH93319; BDU_367.
GeneIDi6918451.
KEGGibdu:BDU_367.
PATRICi20562842. VBIBorDut9941_0394.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciBDUT412419:GJ78-367-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii."
    Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 4:E1000185-E1000185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ly.

Entry informationi

Entry nameiSYE_BORDL
AccessioniPrimary (citable) accession number: B5RLR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 4, 2008
Last modified: February 4, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.