Reviewed, UniProtKB/Swiss-Prot B5RLC9 (MRAY_BORDL)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BDU_306

Organism

Borrelia duttonii (strain Ly) [Complete proteome] [HAMAP]

Taxonomic identifier

412419 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000090594
Regions
	Transmembrane	17 – 37	21	Potential
	Transmembrane	61 – 83	23	Potential
	Transmembrane	88 – 105	18	Potential
	Transmembrane	130 – 150	21	Potential
	Transmembrane	158 – 178	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	230 – 250	21	Potential
	Transmembrane	254 – 274	21	Potential
	Transmembrane	279 – 299	21	Potential
	Transmembrane	328 – 348	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B5RLC9-1 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: 19A99E1AD71F8DC6	FASTA	351	39,558
	10 20 30 40 50 60 MFCFLGLRLL KYITFRTAYA TIFAFLLALI FGPFIISRLK KLKLDQILRK DGPKRHLSEK 70 80 90 100 110 120 MGIPTMGGVL IFFCVLVSLF FWIHFFNIYF LIVLFVMVSF ACLGFTDDLL KIKRKNSDGL 130 140 150 160 170 180 NPKFKIYGQI LFSFISVVML YYFGGEHVSI LYFPFFKSLK LDLGILYIPF GMFVLISASN 190 200 210 220 230 240 SFNLTDGLDG LAIGLSIVVI GALIIIAYLT SRVDFALYLN IPNVKGCEEL VIFLGALLGG 250 260 270 280 290 300 SFGFLWFNAY PAKIMMGDTG SLSIGAVLGM VALILKSEIL FAILAGVFVV ETLSVIIQVV 310 320 330 340 350 VYKKTKKRVF KMAPLHHHFE ELGWSEMQVV IRFWIIGLIF AILALSTIKI R

References

[1]

"The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii." Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 4:E1000185-E1000185(2008) [PubMed: 18787695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000976 Genomic DNA. Translation: ACH93258.1.
RefSeq	YP_002221964.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6918390.
GenomeReviews	Gene locus BDU_306 in contig CP000976_GR.
KEGG	bdu:BDU_306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BORDL

Accession

Primary (citable) accession number: B5RLC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 4, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)