B5RL72 (NTPA_BORDL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BDU_248

Organism

Borrelia duttonii (strain Ly) [Complete proteome] [HAMAP]

Taxonomic identifier

412419 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000145482
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	68 – 69	2	Substrate binding By similarity
Sites
	Metal binding	68	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5RL72 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: AEC4DC7D02D5552C	FASTA	199	23,168
	10 20 30 40 50 60 MKTLFFATSN INKINEVKQI LDIPKIKIEI PQNFDIKETG KTFKENSLLK AKALFKILNN 70 80 90 100 110 120 KQPVFSEDSG LCIEALNMEP GIYSKRYDQY KLGKKLDNNE KNHLIIDLMR EKNNRTAYFI 130 140 150 160 170 180 CVISYIDVDG TINNFEGMLK GTIALNIDYY QKNGFGYDPI FLTTNNKRLS ELNLEEKNKI 190 SHRGIAFDKF KKFLMQFLD

References

[1]

"The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttonii." Lescot M., Audic S., Robert C., Nguyen T.T., Blanc G., Cutler S.J., Wincker P., Couloux A., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 4:E1000185-E1000185(2008) [PubMed: 18787695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ly.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000976 Genomic DNA. Translation: ACH93201.1.
RefSeq	YP_002221907.1. NC_011229.1.
3D structure databases
ProteinModelPortal	B5RL72.
ModBase	Search...
Protein-protein interaction databases
STRING	B5RL72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000001482; EBBORP00000001444; EBBORG00000001482.
GeneID	6918332.
GenomeReviews	Gene locus BDU_248 in contig CP000976_GR.
KEGG	bdu:BDU_248.
PATRIC	20562602. VBIBorDut9941_0275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007496.
HOGENOM	HBG697237.
OMA	YSKRYDQ.
ProtClustDB	PRK14825.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BORDL

Accession

Primary (citable) accession number: B5RL72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 4, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program