B5RGN1 (GHRB_SALG2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate/hydroxypyruvate reductase B
EC=1.1.1.79
EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	SG3787

Organism

Salmonella gallinarum (strain 287/91 / NCTC 13346) [Complete proteome] [HAMAP]

Taxonomic identifier

550538 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella gallinarum

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity. HAMAP MF_01667
Subcellular location	Cytoplasm By similarity HAMAP MF_01667.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: EC hydroxypyruvate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_1000187297
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5RGN1 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: EC2D1D2B9EDEB5D1	FASTA	324	35,255
	10 20 30 40 50 60 MKPSIILYKT LPDDLLHRLE AHFTVTQVPN LHPETVARHA QAFASAQGLL GASETVNRAL 70 80 90 100 110 120 LEKMPALRAA STISVGYDNV EVDALTARKI VLMHTPAVLT ETVADTVMAL MLATARRVVD 130 140 150 160 170 180 VAERVKAGEW TESIGPAWFG VDVHHKTLGI VGMGRIGMAL AQRAHFGFTM PVLYHARRRH 190 200 210 220 230 240 QEAEDRFNAR YCDLDTLLQE ADFVCVILPL TAETRHLFGA TQFARMKSSA IFINAGRGPV 250 260 270 280 290 300 ADENALIAAL QNGEIYAAGL DVFEQEPLSV DSPLLNMSNV VAVPHIGSAT HETRYNMMAC 310 320 AVDNLIDALQ GKIEKNCVNP QAAG

References

[1]

"Comparative genome analysis of Salmonella enteritidis PT4 and Salmonella gallinarum 287/91 provides insights into evolutionary and host adaptation pathways." Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S., Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A., Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B., Ormond D. , Abdellah Z., Brooks K., Cherevach I., Chillingworth T., Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K., Moule S., Mungall K., Saunders M., Whitehead S., Chabalgoity J.A., Maskell D., Humphreys T., Roberts M., Barrow P.A., Dougan G., Parkhill J. Genome Res. 18:1624-1637(2008) [PubMed: 18583645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 287/91 / NCTC 13346.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM933173 Genomic DNA. Translation: CAR39566.1.
RefSeq	YP_002228547.1. NC_011274.1.
3D structure databases
ProteinModelPortal	B5RGN1.
ModBase	Search...
Protein-protein interaction databases
STRING	B5RGN1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6925898.
GenomeReviews	Gene locus SG3787 in contig AM933173_GR.
KEGG	seg:SG3787.
PATRIC	18506317. VBISalEnt1629_4018.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG731446.
OMA	HALTEKK.
ProtClustDB	PRK15409.
Family and domain databases
HAMAP	MF_01667. 2-Hacid_dh_C_GhrB. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023756. Glyo/OHPyrv_Rdtase_B. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K00090.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_SALG2

Accession

Primary (citable) accession number: B5RGN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	November 4, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program