B5RFV5 (B5RFV5_SALG2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G HAMAP-Rule MF_00074
EC=2.1.1.170 HAMAP-Rule MF_00074

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase HAMAP-Rule MF_00074

Gene names

Name:	gidB EMBL CAR39349.1
Synonyms:	rsmG HAMAP-Rule MF_00074
Ordered Locus Names:	SG3560

Organism

Salmonella gallinarum (strain 287/91 / NCTC 13346) [Complete proteome] [HAMAP] EMBL CAR39349.1

Taxonomic identifier

550538 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella gallinarum

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanine in position 527 of 16S rRNA By similarity. HAMAP-Rule MF_00074
Catalytic activity	S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA. HAMAP-Rule MF_00074
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00074 SAAS SAAS003682.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family. HAMAP-Rule MF_00074

Ontologies

Keywords
Biological process	rRNA processing HAMAP-Rule MF_00074 SAAS SAAS003682
Cellular component	Cytoplasm HAMAP-Rule MF_00074 SAAS SAAS003682
Ligand	S-adenosyl-L-methionine HAMAP-Rule MF_00074 SAAS SAAS003682
Molecular function	Methyltransferase HAMAP-Rule MF_00074 SAAS SAAS003682 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	126 – 127	2	S-adenosyl-L-methionine binding By similarity HAMAP-Rule MF_00074
Sites
	Binding site	75	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00074
	Binding site	80	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00074
	Binding site	141	1	S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00074

Sequences

	Sequence		Length	Mass (Da)
	B5RFV5 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: 9A5FABEEDB9F32D5	FASTA	209	23,347
	10 20 30 40 50 60 MTVLNKLSRL LADAGISLTD HQKTLLVAYV DMLHKWNKAY NLTSVRDPNE MLVRHILDSI 70 80 90 100 110 120 VVAPYLQGQR FIDVGTGPGL PGIPLAIVLP DAHFTLLDSL GKRVRFLRQV QHELKLENIT 130 140 150 160 170 180 PVQSRVEAYP SEPPFDGVIS RAFASLNDMV SWCHHLPGEK GRFYALKGQL PGDEIASLPD 190 200 NFSVESVEKL RVPQLEGERH LAIIKSNKV

References

[1]

"Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways." Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S., Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A., Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B., Ormond D. , Abdellah Z., Brooks K., Cherevach I., Chillingworth T., Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K., Moule S., Mungall K., Sanders M., Whitehead S., Chabalgoity J.A., Maskell D., Humphrey T., Roberts M., Barrow P.A., Dougan G., Parkhill J. Genome Res. 18:1624-1637(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 287/91 / NCTC 13346.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM933173 Genomic DNA. Translation: CAR39349.1.
RefSeq	YP_002228345.1. NC_011274.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	550538.SG3560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR39349; CAR39349; SG3560.
GeneID	6923706.
KEGG	seg:SG3560.
PATRIC	18505834. VBISalEnt1629_3779.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0357.
HOGENOM	HOG000221012.
KO	K03501.
OMA	DSHFTLL.
ProtClustDB	PRK00107.
Enzyme and pathway databases
BioCyc	SENT550538:GJ93-3523-MONOMER.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G.
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. gidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B5RFV5_SALG2

Accession

Primary (citable) accession number: B5RFV5

Entry history

Integrated into UniProtKB/TrEMBL:	November 4, 2008
Last sequence update:	November 4, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)