Reviewed, UniProtKB/Swiss-Prot B5REH7 (RLMG_SALG2)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	SG3116

Organism

Salmonella gallinarum (strain 287/91 / NCTC 13346) [Complete proteome] [HAMAP]

Taxonomic identifier

550538 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella gallinarum

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366494

Sequences

	Sequence		Length	Mass (Da)
	B5REH7-1 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: 6C2F1FC4AC2E703D	FASTA	378	42,252
	10 20 30 40 50 60 MSHVDDGFRS LTLKRFPQTD DVNPLLAWEA ADEYLLQQLD ETEIRGPVLI LNDTFGALSC 70 80 90 100 110 120 ALAEHSPYSI GDSYLSELGT RENLRHNGIA ESSVTFLNST ADYPQAPGVV LIKVPKTLAL 130 140 150 160 170 180 LEQQLRALRK VVTAQTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFSH 190 200 210 220 230 240 PQLADAPQTL SWKLEDTGWT IHNHANVFSR TGLDIGARFF MQHLPENLDG EIVDLGCGNG 250 260 270 280 290 300 VIGLSLLAKN PQANVVFVDE SPMAVDSSRL NVETNLPEAF ERCEFMINNA LSGVEPFRFN 310 320 330 340 350 360 AVFCNPPFHQ KHALTDNIAW EMFHHARRCL KINGELYIVA NRHLDYFHKL KKIFGNCATI 370 ATNNKFVILK AVKQGHRR

References

[1]

"Comparative genome analysis of Salmonella enteritidis PT4 and Salmonella gallinarum 287/91 provides insights into evolutionary and host adaptation pathways." Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S., Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A., Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B., Ormond D. , Abdellah Z., Brooks K., Cherevach I., Chillingworth T., Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K., Moule S., Mungall K., Saunders M., Whitehead S., Chabalgoity J.A., Maskell D., Humphreys T., Roberts M., Barrow P.A., Dougan G., Parkhill J. Genome Res. 18:1624-1637(2008) [PubMed: 18583645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM933173 Genomic DNA. Translation: CAR38917.1.
RefSeq	YP_002227935.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6923530.
GenomeReviews	Gene locus SG3116 in contig AM933173_GR.
KEGG	seg:SG3116.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NRHLGYH.
Family and domain databases
HAMAP	MF_01859. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SALG2

Accession

Primary (citable) accession number: B5REH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 4, 2008
Last modified:	November 3, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)