B5R6T3 (PHNX_SALG2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphonoacetaldehyde hydrolase
Short name=Phosphonatase
EC=3.11.1.1

Alternative name(s):
Phosphonoacetaldehyde phosphonohydrolase

Gene names

Name:	phnX
Ordered Locus Names:	SG0443

Organism

Salmonella gallinarum (strain 287/91 / NCTC 13346) [Complete proteome] [HAMAP]

Taxonomic identifier

550538 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella gallinarum

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity.
Catalytic activity	Phosphonoacetaldehyde + H2O = acetaldehyde + phosphate. HAMAP-Rule MF_01375
Cofactor	Binds 1 Mg2+ ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PhnX family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Schiff base
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphonoacetaldehyde hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Phosphonoacetaldehyde hydrolase HAMAP-Rule MF_01375		PRO_1000144839
Sites
	Active site	11	1	Nucleophile By similarity
	Active site	53	1	Schiff-base intermediate with substrate By similarity
	Metal binding	11	1	Magnesium By similarity
	Metal binding	13	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	187	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5R6T3 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: 14FC0778071D940E	FASTA	270	28,783
	10 20 30 40 50 60 MMNRIHAVIL DWAGTTVDFG SFAPTQIFVE AFRQAFDVEI TLAEARVPMG LGKWQHIEAL 70 80 90 100 110 120 GKLPAVDARW QAKFGRSMSA ADIDAIYAAF MPLQIAKVVD FSSPIAGVID TIAALRAEGI 130 140 150 160 170 180 KIGSCSGYPR AVMERLVPAA AEHGYRPDHW VATDDLAAGG RPGPWMALQN VIALGIDAVA 190 200 210 220 230 240 HCVKVDDAAL GISEGLNAGM WTVGLAVSGN EFGATWDAYQ TMSKEDVAVR REHAASKLYA 250 260 270 AGAHYVVDSL ADLSGVIAHI NARLAQGERP

References

[1]

"Comparative genome analysis of Salmonella enteritidis PT4 and Salmonella gallinarum 287/91 provides insights into evolutionary and host adaptation pathways." Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S., Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A., Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B., Ormond D. , Abdellah Z., Brooks K., Cherevach I., Chillingworth T., Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K., Moule S., Mungall K., Saunders M., Whitehead S., Chabalgoity J.A., Maskell D., Humphreys T., Roberts M., Barrow P.A., Dougan G., Parkhill J. Genome Res. 18:1624-1637(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 287/91 / NCTC 13346.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM933173 Genomic DNA. Translation: CAR36342.1.
RefSeq	YP_002225544.1. NC_011274.1.
3D structure databases
ProteinModelPortal	B5R6T3.
ModBase	Search...
Protein-protein interaction databases
STRING	550538.SG0443.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR36342; CAR36342; SG0443.
GeneID	6924425.
KEGG	seg:SG0443.
PATRIC	18499055. VBISalEnt1629_0480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0637.
HOGENOM	HOG000049554.
KO	K05306.
OMA	EFGATWE.
ProtClustDB	PRK13478.
Enzyme and pathway databases
BioCyc	SENT550538:GJ93-440-MONOMER.
Family and domain databases
Gene3D	1.10.150.240. 1 hit. 3.40.50.1000. 2 hits.
HAMAP	MF_01375. PhnX.
InterPro	IPR023214. HAD-like_dom. IPR006402. HAD-SF_hydro_IA_v3. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro. [Graphical view]
Pfam	PF13419. HAD_2. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNX_SALG2

Accession

Primary (citable) accession number: B5R6T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 4, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program