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B5R0E7

- SYM_SALEP

UniProt

B5R0E7 - SYM_SALEP

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Protein

Methionine--tRNA ligase

Gene
metG, SEN2148
Organism
Salmonella enteritidis PT4 (strain P125109)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi146 – 1461Zinc By similarity
Metal bindingi149 – 1491Zinc By similarity
Metal bindingi159 – 1591Zinc By similarity
Metal bindingi162 – 1621Zinc By similarity
Binding sitei336 – 3361ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciSENT550537:GJFI-2177-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Ordered Locus Names:SEN2148
OrganismiSalmonella enteritidis PT4 (strain P125109)
Taxonomic identifieri550537 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000000613: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 677677Methionine--tRNA ligaseUniRule annotationPRO_1000093727Add
BLAST

Proteomic databases

PRIDEiB5R0E7.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi550537.SEN2148.

Structurei

3D structure databases

ProteinModelPortaliB5R0E7.
SMRiB5R0E7. Positions 5-551.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini575 – 677103tRNA-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 2511"HIGH" regionUniRule annotationAdd
BLAST
Motifi333 – 3375"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200400.
OMAiNQCIYVC.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5R0E7-1 [UniParc]FASTAAdd to Basket

« Hide

MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC    50
ADDAHGTPIM LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS 100
DENRELSELI YTRLKENGFI KNRTISQLYD PEKGMFLPDR FVKGTCPKCK 150
SADQYGDNCE VCGATYSPTE LIEPKSVVSG ATPVMRDSEH FFFDLPSFSE 200
MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG FEIPNAPGKY 250
FYVWLDAPIG YMGSFKNLCD KRGDTTSFDE YWKKDSDAEL YHFIGKDIVY 300
FHSLFWPAML EGSHFRKPTN LFVHGYVTVN GAKMSKSRGT FIKASTWLKH 350
FDADSLRYYY TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF 400
INKRFDGVLA AELADPQLYK TFTDAAAVIG EAWESREFGK AIREIMALAD 450
VANRYVDEQA PWVVAKQEGR DADLQAICSM GINLFRVLMT YLKPVLPTLS 500
ERVEAFLNSE LNWDAIEQPL LSHKVNTFKA LYNRIDMKQV EALVEASKEE 550
VKAAAAPVTG PLADFPIQET ITFDDFAKID LRVALIENAE FVEGSDKLLR 600
LTLDLGGEKR NVFSGIRSAY PDPQALIGRQ TVMVANLAPR KMRFGVSEGM 650
VMAAGPGGKD IFLLSPDDGA KPGQQVK 677
Length:677
Mass (Da):76,288
Last modified:November 4, 2008 - v1
Checksum:i948CC4D95594D4FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM933172 Genomic DNA. Translation: CAR33733.1.
RefSeqiYP_002244240.1. NC_011294.1.

Genome annotation databases

EnsemblBacteriaiCAR33733; CAR33733; SEN2148.
PATRICi32334795. VBISalEnt14964_2172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM933172 Genomic DNA. Translation: CAR33733.1 .
RefSeqi YP_002244240.1. NC_011294.1.

3D structure databases

ProteinModelPortali B5R0E7.
SMRi B5R0E7. Positions 5-551.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 550537.SEN2148.

Proteomic databases

PRIDEi B5R0E7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR33733 ; CAR33733 ; SEN2148 .
PATRICi 32334795. VBISalEnt14964_2172.

Phylogenomic databases

eggNOGi COG0143.
HOGENOMi HOG000200400.
OMAi NQCIYVC.
OrthoDBi EOG6CVV9B.

Enzyme and pathway databases

BioCyci SENT550537:GJFI-2177-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00098. Met_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
Pfami PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view ]
PRINTSi PR01041. TRNASYNTHMET.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsi TIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: P125109.

Entry informationi

Entry nameiSYM_SALEP
AccessioniPrimary (citable) accession number: B5R0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 4, 2008
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi