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B5R0E7

- SYM_SALEP

UniProt

B5R0E7 - SYM_SALEP

Protein

Methionine--tRNA ligase

Gene

metG

Organism
Salmonella enteritidis PT4 (strain P125109)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (04 Nov 2008)
      Previous versions | rss
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    Functioni

    Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

    Catalytic activityi

    ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi146 – 1461ZincUniRule annotation
    Metal bindingi149 – 1491ZincUniRule annotation
    Metal bindingi159 – 1591ZincUniRule annotation
    Metal bindingi162 – 1621ZincUniRule annotation
    Binding sitei336 – 3361ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciSENT550537:GJFI-2177-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
    Alternative name(s):
    Methionyl-tRNA synthetaseUniRule annotation
    Short name:
    MetRSUniRule annotation
    Gene namesi
    Name:metGUniRule annotation
    Ordered Locus Names:SEN2148
    OrganismiSalmonella enteritidis PT4 (strain P125109)
    Taxonomic identifieri550537 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000000613: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 677677Methionine--tRNA ligasePRO_1000093727Add
    BLAST

    Proteomic databases

    PRIDEiB5R0E7.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi550537.SEN2148.

    Structurei

    3D structure databases

    ProteinModelPortaliB5R0E7.
    SMRiB5R0E7. Positions 5-551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini575 – 677103tRNA-bindingUniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi15 – 2511"HIGH" regionAdd
    BLAST
    Motifi333 – 3375"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0143.
    HOGENOMiHOG000200400.
    OMAiNQCIYVC.
    OrthoDBiEOG6CVV9B.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    2.20.28.20. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00098. Met_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR004495. Met-tRNA-synth_Ia_bsu_C.
    IPR023458. Met-tRNA_ligase_1.
    IPR014758. Met-tRNA_synth.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR029038. MetRS_Zn.
    IPR012340. NA-bd_OB-fold.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002547. tRNA-bd_dom.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PfamiPF09334. tRNA-synt_1g. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    PRINTSiPR01041. TRNASYNTHMET.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF57770. SSF57770. 1 hit.
    TIGRFAMsiTIGR00398. metG. 1 hit.
    TIGR00399. metG_C_term. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5R0E7-1 [UniParc]FASTAAdd to Basket

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    MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC    50
    ADDAHGTPIM LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS 100
    DENRELSELI YTRLKENGFI KNRTISQLYD PEKGMFLPDR FVKGTCPKCK 150
    SADQYGDNCE VCGATYSPTE LIEPKSVVSG ATPVMRDSEH FFFDLPSFSE 200
    MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG FEIPNAPGKY 250
    FYVWLDAPIG YMGSFKNLCD KRGDTTSFDE YWKKDSDAEL YHFIGKDIVY 300
    FHSLFWPAML EGSHFRKPTN LFVHGYVTVN GAKMSKSRGT FIKASTWLKH 350
    FDADSLRYYY TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF 400
    INKRFDGVLA AELADPQLYK TFTDAAAVIG EAWESREFGK AIREIMALAD 450
    VANRYVDEQA PWVVAKQEGR DADLQAICSM GINLFRVLMT YLKPVLPTLS 500
    ERVEAFLNSE LNWDAIEQPL LSHKVNTFKA LYNRIDMKQV EALVEASKEE 550
    VKAAAAPVTG PLADFPIQET ITFDDFAKID LRVALIENAE FVEGSDKLLR 600
    LTLDLGGEKR NVFSGIRSAY PDPQALIGRQ TVMVANLAPR KMRFGVSEGM 650
    VMAAGPGGKD IFLLSPDDGA KPGQQVK 677
    Length:677
    Mass (Da):76,288
    Last modified:November 4, 2008 - v1
    Checksum:i948CC4D95594D4FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM933172 Genomic DNA. Translation: CAR33733.1.
    RefSeqiYP_002244240.1. NC_011294.1.

    Genome annotation databases

    EnsemblBacteriaiCAR33733; CAR33733; SEN2148.
    PATRICi32334795. VBISalEnt14964_2172.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM933172 Genomic DNA. Translation: CAR33733.1 .
    RefSeqi YP_002244240.1. NC_011294.1.

    3D structure databases

    ProteinModelPortali B5R0E7.
    SMRi B5R0E7. Positions 5-551.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 550537.SEN2148.

    Proteomic databases

    PRIDEi B5R0E7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR33733 ; CAR33733 ; SEN2148 .
    PATRICi 32334795. VBISalEnt14964_2172.

    Phylogenomic databases

    eggNOGi COG0143.
    HOGENOMi HOG000200400.
    OMAi NQCIYVC.
    OrthoDBi EOG6CVV9B.

    Enzyme and pathway databases

    BioCyci SENT550537:GJFI-2177-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    2.20.28.20. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00098. Met_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR004495. Met-tRNA-synth_Ia_bsu_C.
    IPR023458. Met-tRNA_ligase_1.
    IPR014758. Met-tRNA_synth.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR029038. MetRS_Zn.
    IPR012340. NA-bd_OB-fold.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002547. tRNA-bd_dom.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    Pfami PF09334. tRNA-synt_1g. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    PRINTSi PR01041. TRNASYNTHMET.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF57770. SSF57770. 1 hit.
    TIGRFAMsi TIGR00398. metG. 1 hit.
    TIGR00399. metG_C_term. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: P125109.

    Entry informationi

    Entry nameiSYM_SALEP
    AccessioniPrimary (citable) accession number: B5R0E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 4, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3