B5R0E7 (SYM_SALEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	SEN2148

Organism

Salmonella enteritidis PT4 (strain P125109) [Complete proteome] [HAMAP]

Taxonomic identifier

550537 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enteritidis

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_1000093727
Regions
	Domain	575 – 677	103	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	333 – 337	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	162	1	Zinc By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5R0E7 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: 948CC4D95594D4FE	FASTA	677	76,288
	10 20 30 40 50 60 MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC ADDAHGTPIM 70 80 90 100 110 120 LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS DENRELSELI YTRLKENGFI 130 140 150 160 170 180 KNRTISQLYD PEKGMFLPDR FVKGTCPKCK SADQYGDNCE VCGATYSPTE LIEPKSVVSG 190 200 210 220 230 240 ATPVMRDSEH FFFDLPSFSE MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG 250 260 270 280 290 300 FEIPNAPGKY FYVWLDAPIG YMGSFKNLCD KRGDTTSFDE YWKKDSDAEL YHFIGKDIVY 310 320 330 340 350 360 FHSLFWPAML EGSHFRKPTN LFVHGYVTVN GAKMSKSRGT FIKASTWLKH FDADSLRYYY 370 380 390 400 410 420 TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF INKRFDGVLA AELADPQLYK 430 440 450 460 470 480 TFTDAAAVIG EAWESREFGK AIREIMALAD VANRYVDEQA PWVVAKQEGR DADLQAICSM 490 500 510 520 530 540 GINLFRVLMT YLKPVLPTLS ERVEAFLNSE LNWDAIEQPL LSHKVNTFKA LYNRIDMKQV 550 560 570 580 590 600 EALVEASKEE VKAAAAPVTG PLADFPIQET ITFDDFAKID LRVALIENAE FVEGSDKLLR 610 620 630 640 650 660 LTLDLGGEKR NVFSGIRSAY PDPQALIGRQ TVMVANLAPR KMRFGVSEGM VMAAGPGGKD 670 IFLLSPDDGA KPGQQVK

References

[1]

"Comparative genome analysis of Salmonella enteritidis PT4 and Salmonella gallinarum 287/91 provides insights into evolutionary and host adaptation pathways." Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S., Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A., Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B., Ormond D. , Abdellah Z., Brooks K., Cherevach I., Chillingworth T., Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K., Moule S., Mungall K., Saunders M., Whitehead S., Chabalgoity J.A., Maskell D., Humphreys T., Roberts M., Barrow P.A., Dougan G., Parkhill J. Genome Res. 18:1624-1637(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: P125109.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM933172 Genomic DNA. Translation: CAR33733.1.
RefSeq	YP_002244240.1. NC_011294.1.
3D structure databases
ProteinModelPortal	B5R0E7.
SMR	B5R0E7. Positions 5-551.
ModBase	Search...
Protein-protein interaction databases
STRING	550537.SEN2148.
Proteomic databases
PRIDE	B5R0E7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6949668.
KEGG	set:SEN2148.
PATRIC	32334795. VBISalEnt14964_2172.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200400.
KO	K01874.
OMA	RMHGHEV.
ProtClustDB	PRK00133.
Enzyme and pathway databases
BioCyc	SENT550537:GJFI-2177-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_SALEP

Accession

Primary (citable) accession number: B5R0E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 4, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program