B5QWR0 (FUCI_SALEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-fucose isomerase
EC=5.3.1.25

Alternative name(s):
6-deoxy-L-galactose isomerase
FucIase

Gene names

Name:	fucI
Ordered Locus Names:	SEN2820

Organism

Salmonella enteritidis PT4 (strain P125109) [Complete proteome] [HAMAP]

Taxonomic identifier

550537 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enteritidis

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts the aldose L-fucose into the corresponding ketose L-fuculose By similarity. HAMAP-Rule MF_01254
Catalytic activity	L-fucose = L-fuculose. HAMAP-Rule MF_01254
Cofactor	Manganese By similarity.
Pathway	Carbohydrate degradation; L-fucose degradation; L-lactaldehyde and glycerone phosphate from L-fucose: step 1/3. HAMAP-Rule MF_01254
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-fucose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fucose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-fucose isomerase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	L-fucose isomerase HAMAP-Rule MF_01254		PRO_1000139959
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	361	1	Proton acceptor By similarity
	Metal binding	337	1	Manganese By similarity
	Metal binding	361	1	Manganese By similarity
	Metal binding	528	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5QWR0 [UniParc]. Last modified November 4, 2008. Version 1. Checksum: 0A1C6512CFDB846B	FASTA	591	64,789
	10 20 30 40 50 60 MKKISLPKIG IRPVIDGRRM GVRESLEEQT MNMAKATAAL ITEKMRHACG AQVECVIADT 70 80 90 100 110 120 CIAGMAESAA CEEKFSSQNV GVTITVTPCW CYGSETIDMD PMRPKAIWGF NGTERPGAVY 130 140 150 160 170 180 LAAALAAHSQ KGIPAFSIYG HDVQDADDTS IPADVEEKLL RFARAGLAVA SMKGKSYLSV 190 200 210 220 230 240 GGVSMGIAGS IVDHNFFESW LGMKVQAVDM TELRRRIDQK IYDEAELEMA LAWADKNFRY 250 260 270 280 290 300 GEDQNASQYK RNEAQNRAVL KESLLMAMCI RDMMQGNKTL ADKGLVEESL GYNAIAAGFQ 310 320 330 340 350 360 GQRHWTDQYP NGDTAEALLN SSFDWNGVRE PFVVATENDS LNGVAMLFGH QLTGTAQIFA 370 380 390 400 410 420 DVRTYWSPEA VERVTGQALS GLAEHGIIHL INSGSAALDG ACKQRDSEGK PTMKPHWEIS 430 440 450 460 470 480 QQEADACLAA TEWCPAIHEY FRGGGYSSRF LTEGGVPFTM TRVNIIKGLG PVLQIAEGWS 490 500 510 520 530 540 VELPKAMHDQ LDARTNSTWP TTWFAPRLTG KGPFTDVYSV MANWGANHGV LTIGHVGADF 550 560 570 580 590 ITLAAMLRIP VCMHNVEEAK IYRPSAWAAH GMDIEGQDYR ACQNYGPLYK R

References

[1]

"Comparative genome analysis of Salmonella enteritidis PT4 and Salmonella gallinarum 287/91 provides insights into evolutionary and host adaptation pathways." Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S., Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A., Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B., Ormond D. , Abdellah Z., Brooks K., Cherevach I., Chillingworth T., Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K., Moule S., Mungall K., Saunders M., Whitehead S., Chabalgoity J.A., Maskell D., Humphreys T., Roberts M., Barrow P.A., Dougan G., Parkhill J. Genome Res. 18:1624-1637(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: P125109.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM933172 Genomic DNA. Translation: CAR34399.1.
RefSeq	YP_002244892.1. NC_011294.1.
3D structure databases
ProteinModelPortal	B5QWR0.
SMR	B5QWR0. Positions 1-591.
ModBase	Search...
Protein-protein interaction databases
STRING	550537.SEN2820.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6948148.
KEGG	set:SEN2820.
PATRIC	32336231. VBISalEnt14964_2875.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2407.
HOGENOM	HOG000249674.
KO	K01818.
OMA	KIGIRPT.
ProtClustDB	PRK10991.
Enzyme and pathway databases
BioCyc	SENT550537:GJFI-2859-MONOMER.
UniPathway	UPA00563; UER00624.
Family and domain databases
Gene3D	3.20.14.10. 1 hit. 3.40.275.10. 1 hit. 3.40.50.1070. 1 hit.
HAMAP	MF_01254. Fucose_iso.
InterPro	IPR004216. Fuc/Ara_isomerase_C. IPR015888. Fuc_isomerase_C. IPR012888. Fucose_iso_N1. IPR005763. Fucose_isomerase. IPR009015. Fucose_isomerase_N/cen. IPR012889. Fucose_isomerase_N2. [Graphical view]
Pfam	PF02952. Fucose_iso_C. 1 hit. PF07881. Fucose_iso_N1. 1 hit. PF07882. Fucose_iso_N2. 1 hit. [Graphical view]
SUPFAM	SSF50443. Fuc_isomerase_C. 1 hit. SSF53743. Fuc_isomerase_N. 1 hit.
TIGRFAMs	TIGR01089. fucI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FUCI_SALEP

Accession

Primary (citable) accession number: B5QWR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 4, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program