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Protein

UDP-2,3-diacylglucosamine hydrolase

Gene

lpxH

Organism
Salmonella enteritidis PT4 (strain P125109)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + H2O = 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate + UMP.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 Mn2+ ions per subunit in a binuclear metal center.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Manganese 1UniRule annotation1
Metal bindingi10Manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi41Manganese 1UniRule annotation1
Metal bindingi41Manganese 2UniRule annotation1
Metal bindingi79Manganese 2UniRule annotation1
Metal bindingi114Manganese 2; via tele nitrogenUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei160SubstrateUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Binding sitei167SubstrateUniRule annotation1
Metal bindingi195Manganese 2; via pros nitrogenUniRule annotation1
Binding sitei195Substrate; via tele nitrogenUniRule annotation1
Metal bindingi197Manganese 1; via tele nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00480.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-2,3-diacylglucosamine hydrolaseUniRule annotation (EC:3.6.1.54UniRule annotation)
Alternative name(s):
UDP-2,3-diacylglucosamine diphosphataseUniRule annotation
Gene namesi
Name:lpxHUniRule annotation
Ordered Locus Names:SEN0516
OrganismiSalmonella enteritidis PT4 (strain P125109)
Taxonomic identifieri550537 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001295321 – 240UDP-2,3-diacylglucosamine hydrolaseAdd BLAST240

Structurei

3D structure databases

ProteinModelPortaliB5QUU8.
SMRiB5QUU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 80Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the LpxH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000261930.
KOiK03269.
OMAiFDFWFEY.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00575. LpxH. 1 hit.
InterProiView protein in InterPro
IPR024654. Calcineurin-like_PHP_lpxH.
IPR029052. Metallo-depent_PP-like.
IPR010138. UDP-diacylglucosamine_Hdrlase.
PANTHERiPTHR34990:SF3. PTHR34990:SF3. 1 hit.
PfamiView protein in Pfam
PF12850. Metallophos_2. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01854. lipid_A_lpxH. 1 hit.

Sequencei

Sequence statusi: Complete.

B5QUU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLFIADLH LQTEEPAIVA GFLRFLAVEA RQADALYILG DLFEAWIGDD
60 70 80 90 100
DPNPLHREMA VAIKSLVDSG VPCFFIHGNR DFLIGKRFAR ESGMTLLPQE
110 120 130 140 150
KVLDLYGRNV LIMHGDTLCT DDAGYQAFRA KVHNPWVQRL FLTLPLFIRR
160 170 180 190 200
RIAARMRAGS KAANSSKSLD IMDVNAQTVV AEMEKHRVQW LIHGHTHRPA
210 220 230 240
VHELSANDQP AFRVVLGAWH HEGSMVKVTP DNVELIAFPL
Length:240
Mass (Da):26,966
Last modified:November 4, 2008 - v1
Checksum:i14B622934008EC12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM933172 Genomic DNA. Translation: CAR32101.1.
RefSeqiWP_000212289.1. NC_011294.1.

Genome annotation databases

EnsemblBacteriaiCAR32101; CAR32101; SEN0516.
KEGGiset:SEN0516.

Similar proteinsi

Entry informationi

Entry nameiLPXH_SALEP
AccessioniPrimary (citable) accession number: B5QUU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 4, 2008
Last modified: August 30, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families