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Protein

Proteasome subunit beta

Gene

psmB

Organism
Aciduliprofundum boonei (strain DSM 19572 / T469)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81NucleophileUniRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. proteasomal protein catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciABOO439481:GJNL-126-MONOMER.

Protein family/group databases

MEROPSiT01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome beta subunitUniRule annotation
Proteasome core protein PsmBUniRule annotation
Gene namesi
Name:psmBUniRule annotation
Ordered Locus Names:Aboo_0123
ORF Names:ABOONEI_2767, ABOONEI_326
OrganismiAciduliprofundum boonei (strain DSM 19572 / T469)
Taxonomic identifieri439481 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaAciduliprofundum
ProteomesiUP000001400 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 77Removed in mature form; by autocatalysisUniRule annotationPRO_0000397272
Chaini8 – 210203Proteasome subunit betaPRO_0000397273Add
BLAST

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000091083.
KOiK03433.
OMAiGMNEEKT.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_A. Proteasome_B_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5IEE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENAKTGTTT VGIVTKDGVV MGTEHRASME TFIAHKTAQK LYKLDYNIGM
60 70 80 90 100
TTAGLVGDLQ VLVRYMRAEI NLYRIRRKMY MPVGAAATLL SNILNERKFF
110 120 130 140 150
PYYVGLLVGG FDSKGYHVFA IDAAGGAIED KYASVGSGSL FVYGVLEDRW
160 170 180 190 200
KDDLSLDEGI NLVIRGINAA MRRDAASGDG ISVATITKDE GFVELSQEEV
210
EKRMKKLKLK
Length:210
Mass (Da):23,132
Last modified:October 14, 2008 - v1
Checksum:i582EFEE945B50FE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001941 Genomic DNA. Translation: ADD07935.1.
DS990524 Genomic DNA. Translation: EDY35255.1.
DS990523 Genomic DNA. Translation: EDY35319.1.
RefSeqiYP_003482497.1. NC_013926.1.

Genome annotation databases

EnsemblBacteriaiADD07935; ADD07935; Aboo_0123.
EDY35255; EDY35255; ABOONEI_326.
EDY35319; EDY35319; ABOONEI_2767.
GeneIDi8827059.
KEGGiabi:Aboo_0123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001941 Genomic DNA. Translation: ADD07935.1.
DS990524 Genomic DNA. Translation: EDY35255.1.
DS990523 Genomic DNA. Translation: EDY35319.1.
RefSeqiYP_003482497.1. NC_013926.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiT01.002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADD07935; ADD07935; Aboo_0123.
EDY35255; EDY35255; ABOONEI_326.
EDY35319; EDY35319; ABOONEI_2767.
GeneIDi8827059.
KEGGiabi:Aboo_0123.

Phylogenomic databases

HOGENOMiHOG000091083.
KOiK03433.
OMAiGMNEEKT.

Enzyme and pathway databases

BioCyciABOO439481:GJNL-126-MONOMER.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_A. Proteasome_B_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Aciduliprofundum boonei T469."
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Saunders E., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Mikhailova N., Flores G., Reysenbach A.-L., Woyke T.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 19572 / T469.

Entry informationi

Entry nameiPSB_ACIB4
AccessioniPrimary (citable) accession number: B5IEE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 14, 2008
Last modified: April 29, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.