Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

FAD synthase

Gene

ribL

Organism
Aciduliprofundum boonei (strain DSM 19572 / T469)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme.UniRule annotation

Catalytic activityi

ATP + FMN = diphosphate + FAD.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathway:iFAD biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FAD from FMN.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. FAD synthase (ribL)
This subpathway is part of the pathway FAD biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FAD from FMN, the pathway FAD biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201ATP; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 102ATPUniRule annotation
Nucleotide bindingi14 – 174ATPUniRule annotation
Nucleotide bindingi91 – 944ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

Enzyme and pathway databases

BioCyciABOO439481:GJNL-767-MONOMER.
UniPathwayiUPA00277; UER00407.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD synthaseUniRule annotation (EC:2.7.7.2UniRule annotation)
Alternative name(s):
FMN adenylyltransferaseUniRule annotation
Flavin adenine dinucleotide synthaseUniRule annotation
Gene namesi
Name:ribLUniRule annotation
Ordered Locus Names:Aboo_0747
ORF Names:ABOONEI_1934, ABOONEI_2228
OrganismiAciduliprofundum boonei (strain DSM 19572 / T469)
Taxonomic identifieri439481 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaAciduliprofundum
ProteomesiUP000001400 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149FAD synthasePRO_0000406231Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi439481.Aboo_0747.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal FAD synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000284153.
KOiK14656.
OMAiVAHDETV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02115. FAD_synth_arch.
InterProiIPR004821. Cyt_trans-like.
IPR024902. FAD_synth_RibL.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

B5I9H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVMATGVF DILHPGHVLF LREARKLGDE LVVVVARDST VERLKHKPIM
60 70 80 90 100
NEDIRRFMVE SLKPVDRAVL GHKDDMYKTV EDVRPDIIVL GYDQKFDEKE
110 120 130 140
IEEECRKRGI KVKVVRLKKY GDSDLNGTRK IIFKIVDRVD DLYAKDRNS
Length:149
Mass (Da):17,380
Last modified:October 14, 2008 - v1
Checksum:iF820A8DD1EC44056
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251R → K in EDY36241 (PubMed:18445019).Curated
Sequence conflicti53 – 531D → E in EDY36241 (PubMed:18445019).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS990515 Genomic DNA. Translation: EDY37013.1.
DS990517 Genomic DNA. Translation: EDY36241.1.
CP001941 Genomic DNA. Translation: ADD08556.1.
RefSeqiWP_008082530.1. NZ_DS990515.1.
YP_003483118.1. NC_013926.1.

Genome annotation databases

EnsemblBacteriaiADD08556; ADD08556; Aboo_0747.
EDY36241; EDY36241; ABOONEI_2228.
EDY37013; EDY37013; ABOONEI_1934.
GeneIDi8827694.
KEGGiabi:Aboo_0747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS990515 Genomic DNA. Translation: EDY37013.1.
DS990517 Genomic DNA. Translation: EDY36241.1.
CP001941 Genomic DNA. Translation: ADD08556.1.
RefSeqiWP_008082530.1. NZ_DS990515.1.
YP_003483118.1. NC_013926.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi439481.Aboo_0747.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADD08556; ADD08556; Aboo_0747.
EDY36241; EDY36241; ABOONEI_2228.
EDY37013; EDY37013; ABOONEI_1934.
GeneIDi8827694.
KEGGiabi:Aboo_0747.

Phylogenomic databases

HOGENOMiHOG000284153.
KOiK14656.
OMAiVAHDETV.

Enzyme and pathway databases

UniPathwayiUPA00277; UER00407.
BioCyciABOO439481:GJNL-767-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02115. FAD_synth_arch.
InterProiIPR004821. Cyt_trans-like.
IPR024902. FAD_synth_RibL.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Electron microscopy encounters with unusual thermophiles helps direct genomic analysis of Aciduliprofundum boonei."
    Reysenbach A.L., Flores G.E.
    Geobiology 6:331-336(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 19572 / T469.
  2. "Complete sequence of Aciduliprofundum boonei T469."
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Saunders E., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Mikhailova N., Flores G., Reysenbach A.-L., Woyke T.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 19572 / T469.

Entry informationi

Entry nameiRIBL_ACIB4
AccessioniPrimary (citable) accession number: B5I9H4
Secondary accession number(s): B5IC32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 14, 2008
Last modified: June 24, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.