B5FPR2 (K6PF_SALDC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	SeD_A4460

Organism

Salmonella dublin (strain CT_02021853) [Complete proteome] [HAMAP]

Taxonomic identifier

439851 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella dublin

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_1000120051
Regions
	Nucleotide binding	22 – 26	5	ATP By similarity
	Nucleotide binding	155 – 159	5	ATP By similarity
	Nucleotide binding	172 – 188	17	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Metal binding	186	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	188	1	Magnesium By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5FPR2 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 68B0DDFCF689F420	FASTA	320	34,915
	10 20 30 40 50 60 MIKKIGVLTS GGDAPGMNAA IRGVVRAALT EGLEVMGIYD GYLGLYEDRM VQLDRYSVSD 70 80 90 100 110 120 MINRGGTFLG SARFPEFRDE NIRAVAIENL KKRGIDALVV IGGDGSYMGA KRLTEMGFPC 130 140 150 160 170 180 IGLPGTIDND IKGTDYTIGY FTALGTVVEA IDRLRDTSSS HQRISIVEVM GRYCGDLTLA 190 200 210 220 230 240 AAIAGGCEFI VVPEVEFNRE DLVAEIKAGI AKGKKHAIVA ITEHMCDVDE LAHFIEKETG 250 260 270 280 290 300 RETRATVLGH IQRGGSPVPY DRILASRMGA YAIDLLLEGH GGRCVGIQNE QLVHHDIIDA 310 320 IENMKRPFKS DWMECAKKLY

References

[1]

"Complete genome of Salmonella dublin strain CT_02021853." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT_02021853.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001144 Genomic DNA. Translation: ACH75902.1.
RefSeq	YP_002217989.1. NC_011205.1.
3D structure databases
ProteinModelPortal	B5FPR2.
SMR	B5FPR2. Positions 1-320.
ModBase	Search...
Protein-protein interaction databases
STRING	439851.SeD_A4460.
Proteomic databases
PRIDE	B5FPR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACH75902; ACH75902; SeD_A4460.
GeneID	6874055.
KEGG	sed:SeD_A4460.
PATRIC	18496974. VBISalEnt111443_4374.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	EFKTVEG.
ProtClustDB	PRK03202.
Enzyme and pathway databases
BioCyc	SENT439851:GH2Z-4407-MONOMER.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_SALDC

Accession

Primary (citable) accession number: B5FPR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	May 29, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program