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B5FKT0

- THII_SALDC

UniProt

B5FKT0 - THII_SALDC

Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Salmonella dublin (strain CT_02021853)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei265 – 2651ATPUniRule annotation
    Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
    Binding sitei296 – 2961ATPUniRule annotation
    Active sitei456 – 4561Cysteine persulfide intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi183 – 1842ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. thiazole biosynthetic process Source: InterPro
    4. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciSENT439851:GH2Z-459-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:SeD_A0466
    OrganismiSalmonella dublin (strain CT_02021853)
    Taxonomic identifieri439851 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000008322: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482tRNA sulfurtransferasePRO_1000090028Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi344 ↔ 456Redox-activeUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi439851.SeD_A0466.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini61 – 165105THUMPUniRule annotationAdd
    BLAST
    Domaini404 – 48279RhodaneseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 rhodanese domain.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0301.
    HOGENOMiHOG000227469.
    OMAiKLFPEIM.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5FKT0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI    50
    EVRAKDENQR LAIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALAQYRE 100
    QLEGKTFCVR VKRRGKHEFS SIEVERYVGG GLNQHIESAR VKLTNPDVTV 150
    HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV LSLISGGFDS GVSSYMLMRR 200
    GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV AINFEPVVGE 250
    ILEKVDDGQM GVVLKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN 300
    LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS 350
    PTVKAIKAKI EAEEENFDFS ILDKVVEEAN NVDIREIAQQ TQQEVVEVET 400
    VSGFGPNDVI LDIRSVDEQD DKPLKVEGVD VVSLPFYKLS TKFGDLDQSK 450
    TWLLWCERGV MSRLQALYLR EQGFENVKVY RP 482
    Length:482
    Mass (Da):54,861
    Last modified:October 14, 2008 - v1
    Checksum:iC5904AE5D6B17D68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001144 Genomic DNA. Translation: ACH74329.1.
    RefSeqiYP_002214379.1. NC_011205.1.

    Genome annotation databases

    EnsemblBacteriaiACH74329; ACH74329; SeD_A0466.
    PATRICi18489150. VBISalEnt111443_0542.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001144 Genomic DNA. Translation: ACH74329.1 .
    RefSeqi YP_002214379.1. NC_011205.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 439851.SeD_A0466.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACH74329 ; ACH74329 ; SeD_A0466 .
    PATRICi 18489150. VBISalEnt111443_0542.

    Phylogenomic databases

    eggNOGi COG0301.
    HOGENOMi HOG000227469.
    OMAi KLFPEIM.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci SENT439851:GH2Z-459-MONOMER.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    TIGRFAMsi TIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution."
      Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., Leclerc J.E., Ravel J., Cebula T.A.
      J. Bacteriol. 193:3556-3568(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CT_02021853.

    Entry informationi

    Entry nameiTHII_SALDC
    AccessioniPrimary (citable) accession number: B5FKT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3