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B5FGW1

- NAPA_VIBFM

UniProt

B5FGW1 - NAPA_VIBFM

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Vibrio fischeri (strain MJ11)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:VFMJ11_2039
OrganismiVibrio fischeri (strain MJ11)
Taxonomic identifieri388396 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000001857: Chromosome I

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 829800Periplasmic nitrate reductasePRO_1000186378Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi388396.VFMJ11_2039.

Structurei

3D structure databases

ProteinModelPortaliB5FGW1.
SMRiB5FGW1. Positions 41-827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiEYRGKTM.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5FGW1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMTRRAFVK ANAAASAAAV AGVTLPASAT NLIVSSDQTK IKWDKAPCRF
60 70 80 90 100
CGTGCSVLVG TQNGRVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGKDRVQ
110 120 130 140 150
TPMLRMKDGQ YNKDGDFAPV SWDVALDTMA EKWKAALKKH GPTGVGMFGS
160 170 180 190 200
GQWTVMEGYA AVKLMKAGFR SNNIDPNARH CMASAVGAFM RTFGIDEPMG
210 220 230 240 250
CYDDFENADS FVLWGSNMAE MHPVLWTRIS DRRLSHPHVK VNVLSTYYHR
260 270 280 290 300
SFELADKGYI FEPQSDLAIA NFIANYIIQN NAVNWDFVNK HTNFKQATTD
310 320 330 340 350
IGYGLRDDDP IQMEAANPNS GAMSSISFEE YKKSVAPYTA QKASEMSGVS
360 370 380 390 400
EEDLITLAKQ YADPKTKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIA
410 420 430 440 450
TPGNSPFSLT GQPSACGTAR EVGTFSHRLP ADMVVANPKH RAISEKIWKL
460 470 480 490 500
PEGTLNGKPG AHAVVQDRML KDGKINAYWV MCNNNMQAGP NINTERLPGY
510 520 530 540 550
RNPENFIVCS DPYPTATAQA ADLILPTAMW VEKEGAYGNA ERRTQAWYQQ
560 570 580 590 600
VQAKGEAKSD LWQIMEFSKR FKVEEVWGEE LVAKAPEYRG KTMYDILFKN
610 620 630 640 650
GQVDAFPLSE AQELNDDAKA QGFYVQKGLF EEYASFGRGH GHDLAPYDTY
660 670 680 690 700
HTVRGLRWPV VDGKETLWRF KEGSDPYAKK GSDWDFYGKP DGKALIISAP
710 720 730 740 750
YEAPPEVPND EFDMWLCTGR VLEHWHTGTM TRRVPELYKA VPDALCYIHP
760 770 780 790 800
ADAKKRNLRR GDEVLISNKR GEVRVRVETR GRNRPPEGLV FVPFFDARIL
810 820
INKLILDATD PLSKQTDFKK CPVKITKIA
Length:829
Mass (Da):92,594
Last modified:October 14, 2008 - v1
Checksum:i5F9C417D6C0B0950
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001139 Genomic DNA. Translation: ACH65259.1.
RefSeqiYP_002156733.1. NC_011184.1.

Genome annotation databases

EnsemblBacteriaiACH65259; ACH65259; VFMJ11_2039.
GeneIDi6806366.
KEGGivfm:VFMJ11_2039.
PATRICi20122542. VBIVibFis37164_1961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001139 Genomic DNA. Translation: ACH65259.1 .
RefSeqi YP_002156733.1. NC_011184.1.

3D structure databases

ProteinModelPortali B5FGW1.
SMRi B5FGW1. Positions 41-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 388396.VFMJ11_2039.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH65259 ; ACH65259 ; VFMJ11_2039 .
GeneIDi 6806366.
KEGGi vfm:VFMJ11_2039.
PATRICi 20122542. VBIVibFis37164_1961.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi EYRGKTM.
OrthoDBi EOG6CVV7G.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Vibrio fischeri strain MJ11."
    Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MJ11.

Entry informationi

Entry nameiNAPA_VIBFM
AccessioniPrimary (citable) accession number: B5FGW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 14, 2008
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3