Reviewed, UniProtKB/Swiss-Prot B5FGI9 (CYSI_VIBFM)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	VFMJ11_0298

Organism

Vibrio fischeri (strain MJ11) [Complete proteome] [HAMAP]

Taxonomic identifier

388396 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity. HAMAP MF_01540
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_1000146662
Sites
	Metal binding	439	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	445	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	485	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	489	1	Iron (siroheme axial ligand) By similarity
	Metal binding	489	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5FGI9-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 51AF4F9A78C107DA	FASTA	576	64,668
	10 20 30 40 50 60 MSELIDVKTI LSGTELGPLA DNERLKRESK HLRGTIVEDL QDRITGGFTK DNFQLIRFHG 70 80 90 100 110 120 MYQQDDRDIR AERAKQKLEP LHNVMLRARM PGGIITPKQW LAIDKFAEEH TSYGSLRLTT 130 140 150 160 170 180 RQTFQFHGVL KPNIKLMHQT LNSIGIDSIA TAGDVNRNVL CTTNPVESEL HQEAYEWAKK 190 200 210 220 230 240 ISEHLLPKTR AYAEIWLDGE KLETTDEEPI LGSNYLPRKF KTTVVIPPQN DVDVHANDLN 250 260 270 280 290 300 FIAIADNGKL VGFNVLVGGG LAMTHGDTAT YPRKADDFGF VPLKKTLEVA AAVVTTQRDW 310 320 330 340 350 360 GNRSNRKNAK TKYTLDRVGS DVFKAEVEKR AGVQFEESRP YELTERGDRI GWVEGIDGKH 370 380 390 400 410 420 HLALFIENGR LLDYPGKPLK TGVAEIAKVH QGDFRMTANQ NLIVAGVPSE QKDHIEQIAR 430 440 450 460 470 480 AHGLIDDTHS EQRKNSMACV AFPTCPLAMA EAERFLPEFV TEIEGVLKKH NLPEEDNIIF 490 500 510 520 530 540 RVTGCPNGCG RAMLAEIGLV GKAPGRYNFH LGGNRSGTRV PKMYKENITD RQILTEIDHL 550 560 570 VGRWAIERNE NEGFGDFTIR AGIVDEVKVS KRDFHA

References

[1]

"Complete sequence of Vibrio fischeri strain MJ11." Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001139 Genomic DNA. Translation: ACH65130.1.
RefSeq	YP_002155069.1.
3D structure databases
SMR	B5FGI9. Positions 85-574.
ModBase	Search...
Genome annotation databases
GeneID	6807030.
GenomeReviews	Gene locus VFMJ11_0298 in contig CP001139_GR.
KEGG	vfm:VFMJ11_0298.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG626671.
OMA	EEIVIRM.
Family and domain databases
HAMAP	MF_01540. CysI. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_VIBFM

Accession

Primary (citable) accession number: B5FGI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)