B5FG96 (FTHS_VIBFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	VFMJ11_1925

Organism

Vibrio fischeri (strain MJ11) [Complete proteome] [HAMAP]

Taxonomic identifier

388396 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio › Vibrio fischeri

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_1000146710
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5FG96 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: C11574C527FCBFEB	FASTA	582	62,374
	10 20 30 40 50 60 MKSDIEICQT ATLTRMKTIA SNLGLHDDDI TPQGPFKAKV NIDALKRLKS EPNGKLILVS 70 80 90 100 110 120 AITPTPLGEG KTVTTIGLAQ GLAKLGESVS ACIRQPSMGP VFGVKGGAAG GGYSQVAPME 130 140 150 160 170 180 ELNLHLTGDI HAITAAHNLA SAAIDARIYH EQRLGYDVFS EKNQLPALRI DPQRVVWKRV 190 200 210 220 230 240 MDHNDRALRM VTIGKNEDGK TINGYEREDG FDITAASELM AILALATDLQ DLRQRIGRIV 250 260 270 280 290 300 VAYNLDGEPV TTEDLQVAGA MTVTMKFAIN PTLMQTLEGV PTFVHSGPFA NIAHGNSSII 310 320 330 340 350 360 ADNIALKLTD YTVTEGGFGS DMGFEKACNI KAPLSEKSPD CAVLVATLRG IKANSGLFPL 370 380 390 400 410 420 SPGQSLPKEL FAPNKEALDA GLDNLLWHIN NCAKYGLPVV VAINRFPEDT QEELDSLLNW 430 440 450 460 470 480 VSNLDINVDV AISEAFVKGG NGTLELAEKV IKACQQETQF TPLYTSEMSL FDKLNAVAIK 490 500 510 520 530 540 GYGAERIELS EKAQQQLATF EKLGYQSLSV CMAKTPASIS TDGNIKGAPT DFVVPIRELK 550 560 570 580 LCAGAGFIYA LCGNVMTMPG LPEKPAFMNL DIDGDGNIVG LS

References

[1]

"Complete sequence of Vibrio fischeri strain MJ11." Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MJ11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001139 Genomic DNA. Translation: ACH66842.1.
RefSeq	YP_002156619.1. NC_011184.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	388396.VFMJ11_1925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACH66842; ACH66842; VFMJ11_1925.
GeneID	6805121.
KEGG	vfm:VFMJ11_1925.
PATRIC	20122314. VBIVibFis37164_1847.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	AIKPNIM.
ProtClustDB	PRK13506.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_VIBFM

Accession

Primary (citable) accession number: B5FG96

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	May 29, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program