ID   B5FEZ5_ALIFM            Unreviewed;       314 AA.
AC   B5FEZ5;
DT   14-OCT-2008, integrated into UniProtKB/TrEMBL.
DT   14-OCT-2008, sequence version 1.
DT   27-MAR-2024, entry version 98.
DE   RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU003887};
DE            EC=5.4.99.- {ECO:0000256|RuleBase:RU003887};
GN   OrderedLocusNames=VFMJ11_1671 {ECO:0000313|EMBL:ACH65792.1};
OS   Aliivibrio fischeri (strain MJ11) (Vibrio fischeri).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=388396 {ECO:0000313|EMBL:ACH65792.1, ECO:0000313|Proteomes:UP000001857};
RN   [1] {ECO:0000313|Proteomes:UP000001857}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MJ11 {ECO:0000313|Proteomes:UP000001857};
RA   Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S.,
RA   Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.;
RT   "Complete sequence of Vibrio fischeri strain MJ11.";
RL   Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ACH65792.1, ECO:0000313|Proteomes:UP000001857}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MJ11 {ECO:0000313|EMBL:ACH65792.1,
RC   ECO:0000313|Proteomes:UP000001857};
RX   PubMed=19182778; DOI=10.1038/nature07660;
RA   Mandel M.J., Wollenberg M.S., Stabb E.V., Visick K.L., Ruby E.G.;
RT   "A single regulatory gene is sufficient to alter bacterial host range.";
RL   Nature 458:215-218(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2604) in 23S rRNA = pseudouridine(2604) in 23S rRNA;
CC         Xref=Rhea:RHEA:38875, Rhea:RHEA-COMP:10093, Rhea:RHEA-COMP:10094,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.21;
CC         Evidence={ECO:0000256|ARBA:ARBA00036535};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(35) in tRNA(Tyr) = pseudouridine(35) in tRNA(Tyr);
CC         Xref=Rhea:RHEA:60556, Rhea:RHEA-COMP:15607, Rhea:RHEA-COMP:15608,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000256|ARBA:ARBA00036390};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000256|ARBA:ARBA00008348, ECO:0000256|RuleBase:RU003887}.
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DR   EMBL; CP001139; ACH65792.1; -; Genomic_DNA.
DR   RefSeq; WP_005419811.1; NC_011184.1.
DR   AlphaFoldDB; B5FEZ5; -.
DR   GeneID; 77252812; -.
DR   KEGG; vfm:VFMJ11_1671; -.
DR   HOGENOM; CLU_024979_6_1_6; -.
DR   Proteomes; UP000001857; Chromosome I.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02554; PseudoU_synth_RluF; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR   PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR   PANTHER; PTHR47683:SF2; S4 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003887};
KW   RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182};
KW   rRNA processing {ECO:0000256|ARBA:ARBA00022552}.
FT   DOMAIN          9..66
FT                   /note="RNA-binding S4"
FT                   /evidence="ECO:0000259|SMART:SM00363"
FT   REGION          260..314
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        271..314
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   314 AA;  36073 MW;  EBAF57B8A13373C2 CRC64;
     MTSPEPKLTR LNKFISDTGY CSRREADKLI DQQRITINGK IPEMGTKVAD GDEVLVDGKP
     LKEKQDRIYI AFNKPVGITC TTERHVEGNI VDYINHEQRI FPIGRLDKPS DGLIFLTNDG
     DIVNKILRAG NAHEKEYVVR VDKPITDEFL QKMAAGVPIL DTVTLPCKIK QESRFVFRIT
     LTQGLNRQIR RMCEYLDYEV FKLKRVRIMN IDLGKLASGE WRYLSQAEMD QLNGMIGTSS
     KTEEASRKVK EIDGKVRVLK KASSGRNHHS NNTFKENRRE KAKEGRFSKS APKRKNDRKE
     QGKPQSEIKV WRPK
//