Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Vibrio fischeri (strain MJ11)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13UniRule annotation1
Active sitei138UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 106ATPUniRule annotationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processIsoprene biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00094

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:VFMJ11_0801
OrganismiVibrio fischeri (strain MJ11)
Taxonomic identifieri388396 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
Proteomesi
  • UP000001857 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000921241 – 2884-diphosphocytidyl-2-C-methyl-D-erythritol kinaseAdd BLAST288

Proteomic databases

PRIDEiB5FBW7

Structurei

3D structure databases

ProteinModelPortaliB5FBW7
SMRiB5FBW7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000019601
KOiK00919
OMAiRWPSPAK
OrthoDBiPOG091H00XD

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00061 IspE, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR004424 IspE
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PANTHERiPTHR43527:SF2 PTHR43527:SF2, 1 hit
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF010376 IspE, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00154 ispE, 1 hit

Sequencei

Sequence statusi: Complete.

B5FBW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITKTTRWPS PAKLNLFLYI NGQQENGYHE LQTLFQFIDL CDHLTITSNQ
60 70 80 90 100
SGQITLAPDI PGVKTEDNLI WKAATLLQQH SQCEFGAHIE IEKILPMGGG
110 120 130 140 150
IGGGSSNAAT VLIALNFLWQ LNLSNDTLAA LGVKLGADVP IFVHGFAAFA
160 170 180 190 200
EGIGEKLQPA TPKELWYVLI KPEVSIATVD VFTHPDLVRN TPKQPLNALL
210 220 230 240 250
EATYVNDCEK IVRSVYPEVD YQLSWLLEYA PSRLTGTGAC VFAEFNSEKE
260 270 280
AQDVYSLIPD NATGFIARGM NTSPLNRTLE EYKSLCKI
Length:288
Mass (Da):31,741
Last modified:October 14, 2008 - v1
Checksum:iF36B0295FC6A3FBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001139 Genomic DNA Translation: ACH66983.1
RefSeqiWP_012534115.1, NC_011184.1

Genome annotation databases

EnsemblBacteriaiACH66983; ACH66983; VFMJ11_0801
KEGGivfm:VFMJ11_0801

Similar proteinsi

Entry informationi

Entry nameiISPE_VIBFM
AccessioniPrimary (citable) accession number: B5FBW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 14, 2008
Last modified: May 23, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health