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B5FBK7 (SYL_VIBFM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:VFMJ11_0772
OrganismVibrio fischeri (strain MJ11) [Complete proteome] [HAMAP]
Taxonomic identifier388396 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091377

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B5FBK7 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: 31E925A69B10ED41

FASTA85897,020
        10         20         30         40         50         60 
MQEQYNPQDL EQKIQKHWDD NKTFVVTEDA NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRYQRLQG KNVMQPIGWD AFGLPAENAA VKNKTAPAPW TYENIEYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WNREFATCTP EYYRWEQEFF TKLYSKGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
TPVEQKKIPQ WFIKITEYAQ ELLDDLDNLD GWPEMVKTMQ RNWIGRSEGV ELSFAVNGEE 

       250        260        270        280        290        300 
SPLEVYTTRP DTLMGVTYVG IAAGHPLAEK ASQNNPELAA FVEECRNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMDTGLRAI HPLNGREVPV FVANFVLMDY GTGAVMAVPA HDQRDFEFAT KYGLDIIPVI 

       370        380        390        400        410        420 
KPEDGSDLDV SEAAYTEKGV LFDSGEFDGL AFQEAFDAIA AKLEAEGKGK KTVNFRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMVTTEDG EVHPVPADQL PVILPEDVVM DGVTSPIKAD KEWAKTTFNG 

       490        500        510        520        530        540 
EPALRETDTF DTFMESSWYY ARYCSPQADD ILDPEKANYW LPVDQYIGGI EHACMHLLYS 

       550        560        570        580        590        600 
RFFHKLLRDA GYVTSDEPFK QLLCQGMVLA DAFYYTNDKG GKEWVAPTDV TIERDAKGRI 

       610        620        630        640        650        660 
EKAVDDQGRE VEHSGMIKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFASP ADMTLEWQES 

       670        680        690        700        710        720 
GVEGANRFLK RVWKLVHEHT NKGTTEALDT SSLTGDQKAL RRDVHKTIAK VSDDIGRRQT 

       730        740        750        760        770        780 
FNTAIAAIME LMNKLNKAPQ ESAQDRALLD EALKAVVAML YPMTPHASFA MWEALGESDL 

       790        800        810        820        830        840 
DSATWPTFDE NALVEDEKTI VVMINGKLRA KLVVAADATE EHVRELGLKD ENAMKFLDGL 

       850 
TIRKVIYVPG KLLNIVAN 

« Hide

References

[1]"Complete sequence of Vibrio fischeri strain MJ11."
Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MJ11.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001139 Genomic DNA. Translation: ACH65672.1.
RefSeqYP_002155515.1. NC_011184.1.

3D structure databases

ProteinModelPortalB5FBK7.
SMRB5FBK7. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING388396.VFMJ11_0772.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACH65672; ACH65672; VFMJ11_0772.
GeneID6806764.
KEGGvfm:VFMJ11_0772.
PATRIC20120007. VBIVibFis37164_0743.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_VIBFM
AccessionPrimary (citable) accession number: B5FBK7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 14, 2008
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries