B5FBH8 (RLMG_VIBFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	VFMJ11_0743

Organism

Vibrio fischeri (strain MJ11) [Complete proteome] [HAMAP]

Taxonomic identifier

388396 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366533

Sequences

	Sequence		Length	Mass (Da)
	B5FBH8 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 3D29638AA882E1CE	FASTA	382	43,463
	10 20 30 40 50 60 MKTELSLLDR SLNLQRYPKR AQELLQAWDA GDEYIIKYVE EELNLEDGKN ILILNDNFGA 70 80 90 100 110 120 LSCWFSDKHN VTMMTDSFVS QRGTLKNLQR NQCNRVQLIT STEEMPQGFD LVLMQIPKNN 130 140 150 160 170 180 RMLTWQLQQL RQSMDSSCPI IAVNKAKEIH SSTLELFEDY LGETKTSLAW KKHRLVFSNA 190 200 210 220 230 240 NASNPKTIAE AVCWSVDNED IDLLNYPNVY SGEKLDQGAR FMLEHIPSDP ELRHIIDLGC 250 260 270 280 290 300 GNGVLSVKAG QLNPEARITC VDESFMAVES ARRNLEVNLG KERQFQFIAN NCLDGFKKHS 310 320 330 340 350 360 SYLVLCNPPF HQGQAITDHI AWQMFCDAKH ILCKDGKLLV IGNRHLDYDG KLCRLFGEEN 370 380 VTTVASNSKF VILEAVKAEK SK

References

[1]

"Complete sequence of Vibrio fischeri strain MJ11." Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MJ11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001139 Genomic DNA. Translation: ACH66233.1.
RefSeq	YP_002155486.1. NC_011184.1.
3D structure databases
ProteinModelPortal	B5FBH8.
ModBase	Search...
Protein-protein interaction databases
STRING	B5FBH8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6805639.
GenomeReviews	Gene locus VFMJ11_0743 in contig CP001139_GR.
KEGG	vfm:VFMJ11_0743.
PATRIC	20119949. VBIVibFis37164_0714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	CLSK874746.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_VIBFM

Accession

Primary (citable) accession number: B5FBH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program