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B5FAZ8

- B5FAZ8_VIBFM

UniProt

B5FAZ8 - B5FAZ8_VIBFM

Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Vibrio fischeri (strain MJ11)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei679 – 6791ATP; via carbonyl oxygenUniRule annotation
    Metal bindingi719 – 7191MagnesiumUniRule annotation
    Metal bindingi723 – 7231MagnesiumUniRule annotation
    Metal bindingi892 – 8921MagnesiumUniRule annotation
    Active sitei1143 – 11431NucleophileUniRule annotation
    Active sitei1268 – 12681UniRule annotation
    Active sitei1270 – 12701UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi308 – 31912ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotationImported

    Keywords - Biological processi

    Purine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00128.

    Protein family/group databases

    MEROPSiC56.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthaseUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Short name:
    FGAMSUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
    Gene namesi
    Name:purLUniRule annotationImported
    Ordered Locus Names:VFMJ11_0669Imported
    OrganismiVibrio fischeri (strain MJ11)Imported
    Taxonomic identifieri388396 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
    ProteomesiUP000001857: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi388396.VFMJ11_0669.

    Structurei

    3D structure databases

    ProteinModelPortaliB5FAZ8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
    Contains glutamine amidotransferase type-1 domain.SAAS annotation
    In the N-terminal section; belongs to the FGAMS family.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferaseUniRule annotationSAAS annotation

    Phylogenomic databases

    eggNOGiCOG0046.
    HOGENOMiHOG000261359.
    KOiK01952.
    OMAiGEGHAEF.
    OrthoDBiEOG6FNHHR.

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPiMF_00419. PurL_1.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010073. PRibForGlyAmidine_synth.
    IPR022940. PRibForGlyAmidine_synth_bac.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5FAZ8-1 [UniParc]FASTAAdd to Basket

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    MRILRGSPAL SEFRVNKLLE LCRELSLPVT GIYAEFAHFA DVTAELDASE     50
    VQKLEKLLTY GPTIEEHEPE GLLLLTTPRP GTISPWSSKS TDIAHNCGLD 100
    KIARLERGTA FYIESSEELS ELQLVELKAI LHDRMMEVVF TDFESASALF 150
    AVSEPAPYTE VDLLTGGRKA LEDANVTLGL ALAEDEIDYL LESFTEKLER 200
    NPTDIELMMF AQANSEHCRH KIFNADWTID GVKQEKSLFK MIKNTFEVTP 250
    DNVLSAYKDN AAVMTGSTVG RFFPDPKTRQ YNYHQEKTHI LMKVETHNHP 300
    TAISPWPGAS TGSGGEIRDE GATGIGGKPK AGLVAFSVSN LKIPNFVQPW 350
    ETDFGKPSRI VTALDIMLEG PLGGAAFNNE FGRPNLLGYF RTYEEKVNSH 400
    AGEEVRGYHK PIMLAGGLGN IRDEHVQKKE IPVGASLIVL GGPAMNIGLG 450
    GGAASSMDSG SSSEDLDFAS VQRENPEMER RCQEVIDRCW QLGDANPIAF 500
    IHDVGAGGIS NALPELVDDG ERGGIFNLRD VPNDEPGMSP LEIWCNESQE 550
    RYVMAVADKD MATFDAICKR ERAPYAVVGK ATEERELKLE DSHFDNTPID 600
    MPMDILLGKT PKMHRDATTL KVNNPAIDRS GIEMNEAVNR VLRLPTVAEK 650
    TFLITIGDRS VTGLVARDQM VGPWQVPVAN CAVTAASYDS YHGEAMSLGE 700
    RTPVALLDFG ASARLAVGEA ITNIAATNIG DIKHIKLSAN WMSPAGHPGE 750
    DAGLYEAVKA VGEELCPALG LTIPVGKDSM SMKTKWEENG EQKEVTSPLS 800
    LVITAFARVE DVRKTITPQL RTPDNLEGLG NTSLVLIDLG NGKNRLGATA 850
    LAQVYKQLGD KPADVDNAAQ LKGFYEGVQA LVANDQVVAY HDKGDGGLFV 900
    TLAEMAFAGH CGVNANIEAL GEDTLAALFN EELGAVIQVR NDDLDAVLST 950
    LAANGLEACS HVIGSVETSD ELVIRSGEFV VIERNRTELR TIWAETTHKM 1000
    QGLRDNPVCA DQEHEAKKDN SDPGLNVKLS FDVNEDIAAS FINTGAKPKM 1050
    AILREQGVNS HVEMAAAFDR AGFEATDIHM SDILTGQAVL EEYNGLVACG 1100
    GFSYGDVLGA GEGWAKSVLF NDATRDQFEN FFKREDTFSL GVCNGCQMLS 1150
    NLRELIPGAE YWPRFVRNES ERFEARFSLV EVQKSDSVFF NGMEGSRMPI 1200
    AVSHGEGRVE VRDNEHLNAI ENSGTVALRY VDNHGNPTQQ YPNNPNGSPN 1250
    AITGLTTTDG RVTIMMPHPE RVFRTVANSW SPEGWGENGA WMRMFQNARK 1300
    NIG 1303
    Length:1,303
    Mass (Da):142,216
    Last modified:October 14, 2008 - v1
    Checksum:i184AEDF17D7D9344
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001139 Genomic DNA. Translation: ACH65476.1.
    RefSeqiYP_002155412.1. NC_011184.1.

    Genome annotation databases

    EnsemblBacteriaiACH65476; ACH65476; VFMJ11_0669.
    GeneIDi6806582.
    KEGGivfm:VFMJ11_0669.
    PATRICi20119801. VBIVibFis37164_0640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001139 Genomic DNA. Translation: ACH65476.1 .
    RefSeqi YP_002155412.1. NC_011184.1.

    3D structure databases

    ProteinModelPortali B5FAZ8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 388396.VFMJ11_0669.

    Protein family/group databases

    MEROPSi C56.972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACH65476 ; ACH65476 ; VFMJ11_0669 .
    GeneIDi 6806582.
    KEGGi vfm:VFMJ11_0669.
    PATRICi 20119801. VBIVibFis37164_0640.

    Phylogenomic databases

    eggNOGi COG0046.
    HOGENOMi HOG000261359.
    KOi K01952.
    OMAi GEGHAEF.
    OrthoDBi EOG6FNHHR.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPi MF_00419. PurL_1.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010073. PRibForGlyAmidine_synth.
    IPR022940. PRibForGlyAmidine_synth_bac.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR01735. FGAM_synt. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Vibrio fischeri strain MJ11."
      Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
      Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MJ11Imported.

    Entry informationi

    Entry nameiB5FAZ8_VIBFM
    AccessioniPrimary (citable) accession number: B5FAZ8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 14, 2008
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3