Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoribosylformylglycinamidine synthase

Gene

purL

Organism
Vibrio fischeri (strain MJ11)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotationSAAS annotation

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 1 and 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL), Phosphoribosylformylglycinamidine synthase (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 1 and 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL), Phosphoribosylformylglycinamidine synthase (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei679 – 6791ATP; via carbonyl oxygenUniRule annotation
Metal bindingi719 – 7191MagnesiumUniRule annotation
Metal bindingi723 – 7231MagnesiumUniRule annotation
Metal bindingi892 – 8921MagnesiumUniRule annotation
Active sitei1143 – 11431NucleophileUniRule annotation
Active sitei1268 – 12681UniRule annotation
Active sitei1270 – 12701UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi308 – 31912ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotation

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
UPA00074; UER00128.

Protein family/group databases

MEROPSiC56.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthaseUniRule annotationSAAS annotation (EC:6.3.5.3UniRule annotationSAAS annotation)
Short name:
FGAM synthaseUniRule annotation
Short name:
FGAMSUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferaseUniRule annotation
Gene namesi
Name:purLUniRule annotationImported
Ordered Locus Names:VFMJ11_0669Imported
OrganismiVibrio fischeri (strain MJ11)Imported
Taxonomic identifieri388396 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000001857 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB5FAZ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
In the N-terminal section; belongs to the FGAMS family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000261359.
KOiK01952.
OMAiTTVEPFN.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5FAZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILRGSPAL SEFRVNKLLE LCRELSLPVT GIYAEFAHFA DVTAELDASE
60 70 80 90 100
VQKLEKLLTY GPTIEEHEPE GLLLLTTPRP GTISPWSSKS TDIAHNCGLD
110 120 130 140 150
KIARLERGTA FYIESSEELS ELQLVELKAI LHDRMMEVVF TDFESASALF
160 170 180 190 200
AVSEPAPYTE VDLLTGGRKA LEDANVTLGL ALAEDEIDYL LESFTEKLER
210 220 230 240 250
NPTDIELMMF AQANSEHCRH KIFNADWTID GVKQEKSLFK MIKNTFEVTP
260 270 280 290 300
DNVLSAYKDN AAVMTGSTVG RFFPDPKTRQ YNYHQEKTHI LMKVETHNHP
310 320 330 340 350
TAISPWPGAS TGSGGEIRDE GATGIGGKPK AGLVAFSVSN LKIPNFVQPW
360 370 380 390 400
ETDFGKPSRI VTALDIMLEG PLGGAAFNNE FGRPNLLGYF RTYEEKVNSH
410 420 430 440 450
AGEEVRGYHK PIMLAGGLGN IRDEHVQKKE IPVGASLIVL GGPAMNIGLG
460 470 480 490 500
GGAASSMDSG SSSEDLDFAS VQRENPEMER RCQEVIDRCW QLGDANPIAF
510 520 530 540 550
IHDVGAGGIS NALPELVDDG ERGGIFNLRD VPNDEPGMSP LEIWCNESQE
560 570 580 590 600
RYVMAVADKD MATFDAICKR ERAPYAVVGK ATEERELKLE DSHFDNTPID
610 620 630 640 650
MPMDILLGKT PKMHRDATTL KVNNPAIDRS GIEMNEAVNR VLRLPTVAEK
660 670 680 690 700
TFLITIGDRS VTGLVARDQM VGPWQVPVAN CAVTAASYDS YHGEAMSLGE
710 720 730 740 750
RTPVALLDFG ASARLAVGEA ITNIAATNIG DIKHIKLSAN WMSPAGHPGE
760 770 780 790 800
DAGLYEAVKA VGEELCPALG LTIPVGKDSM SMKTKWEENG EQKEVTSPLS
810 820 830 840 850
LVITAFARVE DVRKTITPQL RTPDNLEGLG NTSLVLIDLG NGKNRLGATA
860 870 880 890 900
LAQVYKQLGD KPADVDNAAQ LKGFYEGVQA LVANDQVVAY HDKGDGGLFV
910 920 930 940 950
TLAEMAFAGH CGVNANIEAL GEDTLAALFN EELGAVIQVR NDDLDAVLST
960 970 980 990 1000
LAANGLEACS HVIGSVETSD ELVIRSGEFV VIERNRTELR TIWAETTHKM
1010 1020 1030 1040 1050
QGLRDNPVCA DQEHEAKKDN SDPGLNVKLS FDVNEDIAAS FINTGAKPKM
1060 1070 1080 1090 1100
AILREQGVNS HVEMAAAFDR AGFEATDIHM SDILTGQAVL EEYNGLVACG
1110 1120 1130 1140 1150
GFSYGDVLGA GEGWAKSVLF NDATRDQFEN FFKREDTFSL GVCNGCQMLS
1160 1170 1180 1190 1200
NLRELIPGAE YWPRFVRNES ERFEARFSLV EVQKSDSVFF NGMEGSRMPI
1210 1220 1230 1240 1250
AVSHGEGRVE VRDNEHLNAI ENSGTVALRY VDNHGNPTQQ YPNNPNGSPN
1260 1270 1280 1290 1300
AITGLTTTDG RVTIMMPHPE RVFRTVANSW SPEGWGENGA WMRMFQNARK

NIG
Length:1,303
Mass (Da):142,216
Last modified:October 14, 2008 - v1
Checksum:i184AEDF17D7D9344
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001139 Genomic DNA. Translation: ACH65476.1.
RefSeqiWP_012533077.1. NC_011184.1.
YP_002155412.1. NC_011184.1.

Genome annotation databases

EnsemblBacteriaiACH65476; ACH65476; VFMJ11_0669.
KEGGivfm:VFMJ11_0669.
PATRICi20119801. VBIVibFis37164_0640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001139 Genomic DNA. Translation: ACH65476.1.
RefSeqiWP_012533077.1. NC_011184.1.
YP_002155412.1. NC_011184.1.

3D structure databases

ProteinModelPortaliB5FAZ8.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC56.972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH65476; ACH65476; VFMJ11_0669.
KEGGivfm:VFMJ11_0669.
PATRICi20119801. VBIVibFis37164_0640.

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000261359.
KOiK01952.
OMAiTTVEPFN.
OrthoDBiEOG6FNHHR.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
UPA00074; UER00128.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Vibrio fischeri strain MJ11."
    Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MJ11Imported.

Entry informationi

Entry nameiB5FAZ8_VIBFM
AccessioniPrimary (citable) accession number: B5FAZ8
Entry historyi
Integrated into UniProtKB/TrEMBL: October 14, 2008
Last sequence update: October 14, 2008
Last modified: June 24, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.