B5FA63 (ISPH_VIBFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	VFMJ11_0469

Organism

Vibrio fischeri (strain MJ11) [Complete proteome] [HAMAP]

Taxonomic identifier

388396 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000098986

Sequences

	Sequence		Length	Mass (Da)
	B5FA63 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: A34B9DBBAD2549A9	FASTA	314	34,762
	10 20 30 40 50 60 MKIVLANPRG FCAGVDRAIS IVERALELYE APIYVRHEVV HNRFVVEGLK QRGAIFVEEL 70 80 90 100 110 120 HEVPDDNIVI FSAHGVSQAV RKEAKERALT VFDATCPLVT KVHMEVARAS KKNIEVVLIG 130 140 150 160 170 180 HAGHPEVEGT MGQYASDSAG MYLVETPDDV IKLNVKDPSN LHYVSQTTLS VDETADVIDE 190 200 210 220 230 240 LRRVFPDIQG PRKDDICYAT QNRQDAVRDM ASQVDVMIVV GSKNSSNSNR LRELSEKLGT 250 260 270 280 290 300 TSYLIDCPED LKEEWLTDQA KVGVTAGASA PEELVNQIIE QVKAFGGTAV EELTGREENM 310 FFEVPKELQI KTVS

References

[1]

"Complete sequence of Vibrio fischeri strain MJ11." Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MJ11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001139 Genomic DNA. Translation: ACH66699.1.
RefSeq	YP_002155225.1. NC_011184.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5FA63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6805137.
GenomeReviews	Gene locus VFMJ11_0469 in contig CP001139_GR.
KEGG	vfm:VFMJ11_0469.
PATRIC	20119399. VBIVibFis37164_0454.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
OMA	IVHNTYV.
ProtClustDB	PRK01045.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_VIBFM

Accession

Primary (citable) accession number: B5FA63

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program