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B5FA60

- SYI_VIBFM

UniProt

B5FA60 - SYI_VIBFM

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Vibrio fischeri (strain MJ11)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei576 – 5761Aminoacyl-adenylateUniRule annotation
    Binding sitei620 – 6201ATPUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi935 – 9351ZincUniRule annotation
    Metal bindingi938 – 9381ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:VFMJ11_0466
    OrganismiVibrio fischeri (strain MJ11)
    Taxonomic identifieri388396 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
    ProteomesiUP000001857: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 952952Isoleucine--tRNA ligasePRO_1000189214Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi388396.VFMJ11_0466.

    Structurei

    3D structure databases

    ProteinModelPortaliB5FA60.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi617 – 6215"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5FA60-1 [UniParc]FASTAAdd to Basket

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    MSDYKDTLNL PETGFPMRGN LANREPEMLK RWYDEDLYGE IRKAKKGKKS    50
    FVLHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YVPGWDCHGL 100
    PIELMVEKKK GKPGQKISAA EFREECRKYA AGQVEGQKES FKRLGVMGEW 150
    DKPYRTMDFG TEANIIRSLG KIADQGHLLK GFKPVHWCTD CGSALAEAEV 200
    EYQDKVSPSI DVRFVAADEA ATLAKFSTPE GHQGEGELSV VIWTTTPWTL 250
    PANRAVALHA DLEYVLVQVE AHGEQKAQRL ILASELAKSV LDRANIEHYH 300
    NLGFAKGSDL ELLQFNHPFY NFTVPAILGE HVTTDSGTGI VHTAPGHGQE 350
    DFVVGKKYDL EIANPVGSNG VYLPDTELFA GQHVFKANDS VLEVLKEKGA 400
    LLHHHAYEHS YPHCWRHKTP IIFRATPQWF ISMDQAGLRA KALEEVKSVE 450
    WMPEWGQNRI EGMIEGRPEW CISRQRTWGV PIALFVHKET AELHPDSLEL 500
    IEKVAKLVEE KGIQAWWDVD AAELMGEEDA ANYEKVLDTL DVWFDSGVTH 550
    FSVVDSREEY NFPEEERTHS ADLYLEGSDQ HRGWFQSSLI SSVAMKGKAP 600
    YRQVLTHGFV VDGNGRKMSK SIGNVVAPKD VTNKLGADIL RLWVASTDYT 650
    NEVAVSDEIL KRSADAYRRI RNTARFFLAN LSGFNPATDI VPAEEMVALD 700
    RWAVGRAFAA QQEIIKSYDE YNLHEVTQRL MHFCSIEMGS FYLDVIKDRQ 750
    YTAKKGGHAQ RSCQTALYYI VEALVRWMAP IMSFTADEIW NEMPGEREKF 800
    VFTGEWYQGL FDLAEGEEFN NEFWTDIQAV RASVNKLLEA ARGEKVIGGA 850
    LQAEVTLYAD DALIAKINKL EDELRFVLLT SAATVKPLSE KTESAKATEL 900
    DGLFVDVVAS EAAKCERCWH HVADVGTIEG HEEVCGRCVS NIDGEGEERK 950
    FA 952
    Length:952
    Mass (Da):106,918
    Last modified:October 14, 2008 - v1
    Checksum:i545533062A0E1019
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001139 Genomic DNA. Translation: ACH66418.1.
    RefSeqiYP_002155222.1. NC_011184.1.

    Genome annotation databases

    EnsemblBacteriaiACH66418; ACH66418; VFMJ11_0466.
    GeneIDi6806152.
    KEGGivfm:VFMJ11_0466.
    PATRICi20119393. VBIVibFis37164_0451.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001139 Genomic DNA. Translation: ACH66418.1 .
    RefSeqi YP_002155222.1. NC_011184.1.

    3D structure databases

    ProteinModelPortali B5FA60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 388396.VFMJ11_0466.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACH66418 ; ACH66418 ; VFMJ11_0466 .
    GeneIDi 6806152.
    KEGGi vfm:VFMJ11_0466.
    PATRICi 20119393. VBIVibFis37164_0451.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Vibrio fischeri strain MJ11."
      Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
      Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MJ11.

    Entry informationi

    Entry nameiSYI_VIBFM
    AccessioniPrimary (citable) accession number: B5FA60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3