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B5FA60

- SYI_VIBFM

UniProt

B5FA60 - SYI_VIBFM

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Protein
Isoleucine--tRNA ligase
Gene
ileS, VFMJ11_0466
Organism
Vibrio fischeri (strain MJ11)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761Aminoacyl-adenylate By similarity
Binding sitei620 – 6201ATP By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity
Metal bindingi935 – 9351Zinc By similarity
Metal bindingi938 – 9381Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:VFMJ11_0466
OrganismiVibrio fischeri (strain MJ11)
Taxonomic identifieri388396 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000001857: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189214Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi388396.VFMJ11_0466.

Structurei

3D structure databases

ProteinModelPortaliB5FA60.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi617 – 6215"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5FA60-1 [UniParc]FASTAAdd to Basket

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MSDYKDTLNL PETGFPMRGN LANREPEMLK RWYDEDLYGE IRKAKKGKKS    50
FVLHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YVPGWDCHGL 100
PIELMVEKKK GKPGQKISAA EFREECRKYA AGQVEGQKES FKRLGVMGEW 150
DKPYRTMDFG TEANIIRSLG KIADQGHLLK GFKPVHWCTD CGSALAEAEV 200
EYQDKVSPSI DVRFVAADEA ATLAKFSTPE GHQGEGELSV VIWTTTPWTL 250
PANRAVALHA DLEYVLVQVE AHGEQKAQRL ILASELAKSV LDRANIEHYH 300
NLGFAKGSDL ELLQFNHPFY NFTVPAILGE HVTTDSGTGI VHTAPGHGQE 350
DFVVGKKYDL EIANPVGSNG VYLPDTELFA GQHVFKANDS VLEVLKEKGA 400
LLHHHAYEHS YPHCWRHKTP IIFRATPQWF ISMDQAGLRA KALEEVKSVE 450
WMPEWGQNRI EGMIEGRPEW CISRQRTWGV PIALFVHKET AELHPDSLEL 500
IEKVAKLVEE KGIQAWWDVD AAELMGEEDA ANYEKVLDTL DVWFDSGVTH 550
FSVVDSREEY NFPEEERTHS ADLYLEGSDQ HRGWFQSSLI SSVAMKGKAP 600
YRQVLTHGFV VDGNGRKMSK SIGNVVAPKD VTNKLGADIL RLWVASTDYT 650
NEVAVSDEIL KRSADAYRRI RNTARFFLAN LSGFNPATDI VPAEEMVALD 700
RWAVGRAFAA QQEIIKSYDE YNLHEVTQRL MHFCSIEMGS FYLDVIKDRQ 750
YTAKKGGHAQ RSCQTALYYI VEALVRWMAP IMSFTADEIW NEMPGEREKF 800
VFTGEWYQGL FDLAEGEEFN NEFWTDIQAV RASVNKLLEA ARGEKVIGGA 850
LQAEVTLYAD DALIAKINKL EDELRFVLLT SAATVKPLSE KTESAKATEL 900
DGLFVDVVAS EAAKCERCWH HVADVGTIEG HEEVCGRCVS NIDGEGEERK 950
FA 952
Length:952
Mass (Da):106,918
Last modified:October 14, 2008 - v1
Checksum:i545533062A0E1019
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001139 Genomic DNA. Translation: ACH66418.1.
RefSeqiYP_002155222.1. NC_011184.1.

Genome annotation databases

EnsemblBacteriaiACH66418; ACH66418; VFMJ11_0466.
GeneIDi6806152.
KEGGivfm:VFMJ11_0466.
PATRICi20119393. VBIVibFis37164_0451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001139 Genomic DNA. Translation: ACH66418.1 .
RefSeqi YP_002155222.1. NC_011184.1.

3D structure databases

ProteinModelPortali B5FA60.
ModBasei Search...

Protein-protein interaction databases

STRINGi 388396.VFMJ11_0466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH66418 ; ACH66418 ; VFMJ11_0466 .
GeneIDi 6806152.
KEGGi vfm:VFMJ11_0466.
PATRICi 20119393. VBIVibFis37164_0451.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Vibrio fischeri strain MJ11."
    Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MJ11.

Entry informationi

Entry nameiSYI_VIBFM
AccessioniPrimary (citable) accession number: B5FA60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 14, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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