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B5FA60

- SYI_VIBFM

UniProt

B5FA60 - SYI_VIBFM

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Vibrio fischeri (strain MJ11)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761Aminoacyl-adenylateUniRule annotation
Binding sitei620 – 6201ATPUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi935 – 9351ZincUniRule annotation
Metal bindingi938 – 9381ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:VFMJ11_0466
OrganismiVibrio fischeri (strain MJ11)
Taxonomic identifieri388396 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000001857: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952Isoleucine--tRNA ligasePRO_1000189214Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi388396.VFMJ11_0466.

Structurei

3D structure databases

ProteinModelPortaliB5FA60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi617 – 6215"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5FA60 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKDTLNL PETGFPMRGN LANREPEMLK RWYDEDLYGE IRKAKKGKKS
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELMVEKKK GKPGQKISAA EFREECRKYA AGQVEGQKES FKRLGVMGEW
160 170 180 190 200
DKPYRTMDFG TEANIIRSLG KIADQGHLLK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYQDKVSPSI DVRFVAADEA ATLAKFSTPE GHQGEGELSV VIWTTTPWTL
260 270 280 290 300
PANRAVALHA DLEYVLVQVE AHGEQKAQRL ILASELAKSV LDRANIEHYH
310 320 330 340 350
NLGFAKGSDL ELLQFNHPFY NFTVPAILGE HVTTDSGTGI VHTAPGHGQE
360 370 380 390 400
DFVVGKKYDL EIANPVGSNG VYLPDTELFA GQHVFKANDS VLEVLKEKGA
410 420 430 440 450
LLHHHAYEHS YPHCWRHKTP IIFRATPQWF ISMDQAGLRA KALEEVKSVE
460 470 480 490 500
WMPEWGQNRI EGMIEGRPEW CISRQRTWGV PIALFVHKET AELHPDSLEL
510 520 530 540 550
IEKVAKLVEE KGIQAWWDVD AAELMGEEDA ANYEKVLDTL DVWFDSGVTH
560 570 580 590 600
FSVVDSREEY NFPEEERTHS ADLYLEGSDQ HRGWFQSSLI SSVAMKGKAP
610 620 630 640 650
YRQVLTHGFV VDGNGRKMSK SIGNVVAPKD VTNKLGADIL RLWVASTDYT
660 670 680 690 700
NEVAVSDEIL KRSADAYRRI RNTARFFLAN LSGFNPATDI VPAEEMVALD
710 720 730 740 750
RWAVGRAFAA QQEIIKSYDE YNLHEVTQRL MHFCSIEMGS FYLDVIKDRQ
760 770 780 790 800
YTAKKGGHAQ RSCQTALYYI VEALVRWMAP IMSFTADEIW NEMPGEREKF
810 820 830 840 850
VFTGEWYQGL FDLAEGEEFN NEFWTDIQAV RASVNKLLEA ARGEKVIGGA
860 870 880 890 900
LQAEVTLYAD DALIAKINKL EDELRFVLLT SAATVKPLSE KTESAKATEL
910 920 930 940 950
DGLFVDVVAS EAAKCERCWH HVADVGTIEG HEEVCGRCVS NIDGEGEERK

FA
Length:952
Mass (Da):106,918
Last modified:October 14, 2008 - v1
Checksum:i545533062A0E1019
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001139 Genomic DNA. Translation: ACH66418.1.
RefSeqiYP_002155222.1. NC_011184.1.

Genome annotation databases

EnsemblBacteriaiACH66418; ACH66418; VFMJ11_0466.
GeneIDi6806152.
KEGGivfm:VFMJ11_0466.
PATRICi20119393. VBIVibFis37164_0451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001139 Genomic DNA. Translation: ACH66418.1 .
RefSeqi YP_002155222.1. NC_011184.1.

3D structure databases

ProteinModelPortali B5FA60.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 388396.VFMJ11_0466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACH66418 ; ACH66418 ; VFMJ11_0466 .
GeneIDi 6806152.
KEGGi vfm:VFMJ11_0466.
PATRICi 20119393. VBIVibFis37164_0451.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Vibrio fischeri strain MJ11."
    Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MJ11.

Entry informationi

Entry nameiSYI_VIBFM
AccessioniPrimary (citable) accession number: B5FA60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 14, 2008
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3