B5F9W4 (LPXA_VIBFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Short name=UDP-N-acetylglucosamine acyltransferase
EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	VFMJ11_2084

Organism

Vibrio fischeri (strain MJ11) [Complete proteome] [HAMAP]

Taxonomic identifier

388396 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio › Vibrio fischeri

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine. HAMAP-Rule MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP-Rule MF_00387
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP-Rule MF_00387		PRO_1000190866

Sequences

	Sequence		Length	Mass (Da)
	B5F9W4 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 94641B982947DB17	FASTA	262	28,288
	10 20 30 40 50 60 MIHETAKIHP SAVIEGNVTI EANVSVGPFT YISGNVTIGE GTEVMSHVVI KGDTTIGKDN 70 80 90 100 110 120 RIFAFAIIGE ESQDKKYGGE ATTVVIGDRN VIRESVQIHR GTVQDRGVTT VGSDNLLCVN 130 140 150 160 170 180 VHIAHDCVVG DNIIMGNNAT LAGHVTVEDF AIVSALSPVH QFCTVGAHSF IGGASVVVQD 190 200 210 220 230 240 VPPFVMAQGN HCKPFGINIE GLKRRGFEKA EIHAIRRAYK ALYRNGNTLE EAKVEINKEI 250 260 EAFPVLQGFL DLFEKSTRGI IR

References

[1]

"Complete sequence of Vibrio fischeri strain MJ11." Mandel M.J., Stabb E.V., Ruby E.G., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MJ11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001139 Genomic DNA. Translation: ACH66581.1.
RefSeq	YP_002156778.1. NC_011184.1.
3D structure databases
ProteinModelPortal	B5F9W4.
SMR	B5F9W4. Positions 1-262.
ModBase	Search...
Protein-protein interaction databases
STRING	388396.VFMJ11_2084.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACH66581; ACH66581; VFMJ11_2084.
GeneID	6805316.
KEGG	vfm:VFMJ11_2084.
PATRIC	20122632. VBIVibFis37164_2006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1043.
HOGENOM	HOG000294326.
KO	K00677.
OMA	REFCTFN.
ProtClustDB	PRK05289.
Enzyme and pathway databases
UniPathway	UPA00359; UER00477.
Family and domain databases
HAMAP	MF_00387. LpxA.
InterPro	IPR001451. Hexapep_transf. IPR010137. Lipid_A_LpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 3 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_VIBFM

Accession

Primary (citable) accession number: B5F9W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program