Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Salmonella agona (strain SL483)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nucleotidyltransferase, Transferase
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:SeAg_B0253
OrganismiSalmonella agona (strain SL483)
Taxonomic identifieri454166 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000008819 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001147591 – 890Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST890

Structurei

3D structure databases

ProteinModelPortaliB5F8S8.
SMRiB5F8S8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini468 – 590HDPROSITE-ProRule annotationAdd BLAST123
Domaini709 – 784ACT 1UniRule annotationAdd BLAST76
Domaini816 – 890ACT 2UniRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 349UridylyltransferaseAdd BLAST349
Regioni350 – 708Uridylyl-removingAdd BLAST359

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000261778.
KOiK00990.
OMAiARICGYF.

Family and domain databases

CDDicd00077. HDc. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR002934. Polymerase_NTP_transf_dom.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF52. PTHR13734:SF52. 1 hit.
PfamiView protein in Pfam
PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.
PS51831. HD. 1 hit.

Sequencei

Sequence statusi: Complete.

B5F8S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLPEQHAN TALPTLPDQP QNPGVWPRAE LTVAGIKARI DIFQHWLGEA
60 70 80 90 100
FDSGICAEQL IEARTEFIDQ LLQRLWIEAG FGQIADLALV AVGGYGRGEL
110 120 130 140 150
HPLSDIDLLI LSRKKLPDEQ AQKVGELLTL LWDVKLDVGH SVRTLEECLL
160 170 180 190 200
EGLSDLTVAT NLIETRLLIG DVALFLALQK HIFSEGFWPS DKFYAAKVEE
210 220 230 240 250
QNQRHQRYHG TSYNLEPDIK SSPGGLRDIH TLQWVARRHF GATSLDEMVG
260 270 280 290 300
FGFLTPAERA ELNECLHILW RIRFALHLVV SRYDNRLLFD RQLSVAQRLN
310 320 330 340 350
YSGEGNDPVE RMMKDYFRVT RRVSELNQML LQLFDEAILA LPADEKPRPV
360 370 380 390 400
DDEFQLRGTL IDLRDDTLFI REPQAILRMF YMMVRNSAIT GIYSTTLRHL
410 420 430 440 450
RHARRHLSQP LCYIPEARTL FLSMLRHPGA VSRGLLPMHR HSVLWAYMPQ
460 470 480 490 500
WSHIVGQMQF DLFHAYTVDE HTIRVMLKLE SFAKEETRQR HPLCVDLWPR
510 520 530 540 550
LPHPELILIA ALFHDIAKGR GGDHSVLGAQ DVLTFAELHG LNSRETQLVA
560 570 580 590 600
WLVRQHLLMS VTAQRRDIQD PEVIKQFAEE VQTETRLRFL VCLTVADICA
610 620 630 640 650
TNETLWNSWK QSLLRELYFA TEKQLRRGMQ NTPDMRERVR HHQLQALALL
660 670 680 690 700
RMDNIDEAAL HKIWTRCRAN YFVRHSPNQL AWHARHLLQH DLSQPLVLLS
710 720 730 740 750
PQATRGGTEI FIWSPDRPYL FAAVCAELDR RNLSVHDAQI FTTRDGMAMD
760 770 780 790 800
TFIVLEPDGN PLAADRHDVI RTGLEQTITQ RSWQPPQPRR QPAKLRHFTV
810 820 830 840 850
ETEVNFLPTH TDRKSFMELI ALDQPGLLAR VGQIFADLGI SLHGARITTI
860 870 880 890
GERVEDLFII ATADRRALNN VLQLEVQQRL TAALNPNDKG
Length:890
Mass (Da):102,222
Last modified:October 14, 2008 - v1
Checksum:i313D763DCCF77460
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001138 Genomic DNA. Translation: ACH49835.1.
RefSeqiWP_001094522.1. NC_011149.1.

Genome annotation databases

EnsemblBacteriaiACH49835; ACH49835; SeAg_B0253.
KEGGisea:SeAg_B0253.

Similar proteinsi

Entry informationi

Entry nameiGLND_SALA4
AccessioniPrimary (citable) accession number: B5F8S8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: October 14, 2008
Last modified: September 27, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families