B5F8N4 (RTCA_SALA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	SeAg_B3719

Organism

Salmonella agona (strain SL483) [Complete proteome] [HAMAP]

Taxonomic identifier

454166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella enterica subsp. enterica serovar Agona

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000099351
Regions
	Nucleotide binding	283 – 287	5	ATP By similarity
Sites
	Active site	308	1	Tele-AMP-histidine intermediate By similarity
	Binding site	103	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5F8N4 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 9C651286F71ECBAB	FASTA	344	36,124
	10 20 30 40 50 60 MARIIALDGA QGEGGGQILR SALSLSMITG QPFEMSGIRA GRAKPGLLRQ HLTAVRAATE 70 80 90 100 110 120 ICGAQVNGDE LGSQQLRFTP GPIRGGEYRF AIGSAGSCML VLQTVLPALW FADGSSRVEV 130 140 150 160 170 180 HGGTHNQAAP SADFICRVWE PLLARMGINQ RTTLIKHGFY PAGGGAAATV VEPAASLRGL 190 200 210 220 230 240 TLISRGETRR ITAEALLASV PHHVGEREVA TLEAHFPRAE KNVVALEGGC GPGNALSLMI 250 260 270 280 290 300 QSEQLTELFA AFGVKGTSAE AVANQVAHEA RRYLASPAAV GEHLADQLIL PLALAGEGAF 310 320 330 340 TVARASAHLL TNIAVVERFL PVTFNLEEAR EMVQVMVSKK DSGS

References

[1]	"Complete genome of Salmonella agona strain SL483." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL483.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001138 Genomic DNA. Translation: ACH51093.1.
RefSeq	YP_002148441.1. NC_011149.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5F8N4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6793166.
GenomeReviews	Gene locus SeAg_B3719 in contig CP001138_GR.
KEGG	sea:SeAg_B3719.
PATRIC	18485676. VBISalEnt65316_3614.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG683539.
OMA	GGTDVAW.
ProtClustDB	PRK04204.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF52913. RNA3'-term_phos_cycl_insert. 1 hit. SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 2 hits.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_SALA4

Accession

Primary (citable) accession number: B5F8N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program