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B5F7R5 (RSMB_SALA4) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase B

EC=2.1.1.176
Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB
Gene names
Name:rsmB
Synonyms:sun
Ordered Locus Names:SeAg_B3604
OrganismSalmonella agona (strain SL483) [Complete proteome] [HAMAP]
Taxonomic identifier454166 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity. HAMAP-Rule MF_01856

Catalytic activity

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA. HAMAP-Rule MF_01856

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01856.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA (cytosine) methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Ribosomal RNA small subunit methyltransferase B HAMAP-Rule MF_01856
PRO_0000366163

Regions

Region254 – 2607S-adenosyl-L-methionine binding By similarity

Sites

Active site3751Nucleophile By similarity
Binding site2771S-adenosyl-L-methionine By similarity
Binding site3031S-adenosyl-L-methionine By similarity
Binding site3221S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
B5F7R5 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: 242E706DB290E8F7

FASTA42948,070
        10         20         30         40         50         60 
MKKQNNLRSL AAQAVEQVVE QGQSLSNVLP PLQQKVADKD KALLQELCFG VLRTLSQLEW 

        70         80         90        100        110        120 
LINKLMSRPM TGKQRTVHYL IMVGFYQLLY TRVPPHAALA ETVEGAVSIK RPQLKGLING 

       130        140        150        160        170        180 
VLRQFQRQQE TLLNEFATSD ARFLHPGWLV KRLQNAYPTQ WQRIIEANNQ RPPMWLRVNR 

       190        200        210        220        230        240 
THHTRDGWLG LLEDAGMKGY PHPDYPDAVR LETPAPVHAL PGFAEGWVTV QDASAQGCAV 

       250        260        270        280        290        300 
FLAPQNGEHI LDLCAAPGGK TTHILEVAPE ADVLAVDIDE QRLSRVYDNL KRLGMKATVK 

       310        320        330        340        350        360 
QGDGRYPAQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIAELA QLQAEILDAV 

       370        380        390        400        410        420 
WPRLNPGGTL VYATCSVLPE ENRDQIKAFL QRTPDAALSE TGTPDQPGQQ NLPGGEEGDG 


FFYAKLIKK 

« Hide

References

[1]"Complete genome of Salmonella agona strain SL483."
Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SL483.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001138 Genomic DNA. Translation: ACH51072.1.
RefSeqYP_002148329.1. NC_011149.1.

3D structure databases

ProteinModelPortalB5F7R5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING454166.SeAg_B3604.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACH51072; ACH51072; SeAg_B3604.
PATRIC18485450. VBISalEnt65316_3501.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0144.
HOGENOMHOG000037300.
OMAGHDGFYY.
OrthoDBEOG6091D0.
ProtClustDBPRK10901.

Enzyme and pathway databases

BioCycSENT454166:GHBA-3576-MONOMER.

Family and domain databases

Gene3D1.10.940.10. 1 hit.
HAMAPMF_01856. 16SrRNA_methyltr_B.
InterProIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
[Graphical view]
PfamPF01189. Nol1_Nop2_Fmu. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSPR02008. RCMTFAMILY.
SUPFAMSSF48013. SSF48013. 1 hit.
TIGRFAMsTIGR00563. rsmB. 1 hit.
PROSITEPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMB_SALA4
AccessionPrimary (citable) accession number: B5F7R5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: October 14, 2008
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families