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Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Salmonella agona (strain SL483)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei277 – 2771S-adenosyl-L-methionineUniRule annotation
Binding sitei303 – 3031S-adenosyl-L-methionineUniRule annotation
Binding sitei322 – 3221S-adenosyl-L-methionineUniRule annotation
Active sitei375 – 3751NucleophileUniRule annotation

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. rRNA (cytosine) methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSENT454166:GHBA-3576-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase BUniRule annotation (EC:2.1.1.176UniRule annotation)
Alternative name(s):
16S rRNA m5C967 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmBUniRule annotation
Gene namesi
Name:rsmBUniRule annotation
Synonyms:sunUniRule annotation
Ordered Locus Names:SeAg_B3604
OrganismiSalmonella agona (strain SL483)
Taxonomic identifieri454166 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000008819 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Ribosomal RNA small subunit methyltransferase BPRO_0000366163Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi454166.SeAg_B3604.

Structurei

3D structure databases

ProteinModelPortaliB5F7R5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 2607S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0144.
HOGENOMiHOG000037300.
OMAiLRVNRQH.
OrthoDBiEOG6091D0.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Nol1_Nop2_Fmu. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5F7R5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQNNLRSL AAQAVEQVVE QGQSLSNVLP PLQQKVADKD KALLQELCFG
60 70 80 90 100
VLRTLSQLEW LINKLMSRPM TGKQRTVHYL IMVGFYQLLY TRVPPHAALA
110 120 130 140 150
ETVEGAVSIK RPQLKGLING VLRQFQRQQE TLLNEFATSD ARFLHPGWLV
160 170 180 190 200
KRLQNAYPTQ WQRIIEANNQ RPPMWLRVNR THHTRDGWLG LLEDAGMKGY
210 220 230 240 250
PHPDYPDAVR LETPAPVHAL PGFAEGWVTV QDASAQGCAV FLAPQNGEHI
260 270 280 290 300
LDLCAAPGGK TTHILEVAPE ADVLAVDIDE QRLSRVYDNL KRLGMKATVK
310 320 330 340 350
QGDGRYPAQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIAELA
360 370 380 390 400
QLQAEILDAV WPRLNPGGTL VYATCSVLPE ENRDQIKAFL QRTPDAALSE
410 420
TGTPDQPGQQ NLPGGEEGDG FFYAKLIKK
Length:429
Mass (Da):48,070
Last modified:October 14, 2008 - v1
Checksum:i242E706DB290E8F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001138 Genomic DNA. Translation: ACH51072.1.
RefSeqiYP_002148329.1. NC_011149.1.

Genome annotation databases

EnsemblBacteriaiACH51072; ACH51072; SeAg_B3604.
PATRICi18485450. VBISalEnt65316_3501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001138 Genomic DNA. Translation: ACH51072.1.
RefSeqiYP_002148329.1. NC_011149.1.

3D structure databases

ProteinModelPortaliB5F7R5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi454166.SeAg_B3604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH51072; ACH51072; SeAg_B3604.
PATRICi18485450. VBISalEnt65316_3501.

Phylogenomic databases

eggNOGiCOG0144.
HOGENOMiHOG000037300.
OMAiLRVNRQH.
OrthoDBiEOG6091D0.

Enzyme and pathway databases

BioCyciSENT454166:GHBA-3576-MONOMER.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Nol1_Nop2_Fmu. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution."
    Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., Leclerc J.E., Ravel J., Cebula T.A.
    J. Bacteriol. 193:3556-3568(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SL483.

Entry informationi

Entry nameiRSMB_SALA4
AccessioniPrimary (citable) accession number: B5F7R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: October 14, 2008
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.