B5F3J7 (RUVB_SALA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:	ruvB
Ordered Locus Names:	SeAg_B1230

Organism

Salmonella agona (strain SL483) [Complete proteome] [HAMAP]

Taxonomic identifier

454166 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Agona

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing By similarity. HAMAP-Rule MF_00016
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00016
Subunit structure	Forms a complex with RuvA By similarity.
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Holliday junction ATP-dependent DNA helicase RuvB HAMAP-Rule MF_00016		PRO_1000089671
Regions
	Nucleotide binding	62 – 69	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B5F3J7 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: D154B684EE5DBC54	FASTA	336	37,019
	10 20 30 40 50 60 MIEADRLISA GATIAEDVAD RAIRPKLLAE YVGQPQVRSQ MEIFIQAAKR RGDALDHLLI 70 80 90 100 110 120 FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM LTNLEPHDVL FIDEIHRLSP 130 140 150 160 170 180 VVEEVLYPAM EDYQLDIMIG EGPAARSIKI DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR 190 200 210 220 230 240 LEFYQVPDLQ HIVGRSARHM GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG 250 260 270 280 290 300 AISAEIAAQA LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV 310 320 330 LEPYLIQQGF LQRTPRGRMA TVRAWNHFGI TPPEMP

References

[1]	"Complete genome of Salmonella agona strain SL483." Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SL483.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001138 Genomic DNA. Translation: ACH48707.1.
RefSeq	YP_002146129.1. NC_011149.1.
3D structure databases
ProteinModelPortal	B5F3J7.
SMR	B5F3J7. Positions 22-333.
ModBase	Search...
Protein-protein interaction databases
STRING	454166.SeAg_B1230.
Proteomic databases
PRIDE	B5F3J7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACH48707; ACH48707; SeAg_B1230.
GeneID	6797230.
KEGG	sea:SeAg_B1230.
PATRIC	18480789. VBISalEnt65316_1212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2255.
HOGENOM	HOG000218623.
KO	K03551.
OMA	HIIAKEM.
ProtClustDB	PRK00080.
Enzyme and pathway databases
BioCyc	SENT454166:GHBA-263-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00016. DNA_helic_RuvB.
InterPro	IPR003593. AAA+_ATPase. IPR004605. DNA_helicase_Holl-junc_RuvB. IPR008823. DNA_helicase_Holl-junc_RuvB_C. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13779:SF2. PTHR13779:SF2. 1 hit.
Pfam	PF05491. RuvB_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
ProDom	PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00635. ruvB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVB_SALA4

Accession

Primary (citable) accession number: B5F3J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program