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B5F291 (B5F291_SALA4) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Signal recognition particle protein HAMAP-Rule MF_00306
Alternative name(s):
Fifty-four homolog HAMAP-Rule MF_00306
Gene names
Name:ffh HAMAP-Rule MF_00306 EMBL ACH48818.1
Ordered Locus Names:SeAg_B2823 EMBL ACH48818.1
OrganismSalmonella agona (strain SL483) [Complete proteome] [HAMAP] EMBL ACH48818.1
Taxonomic identifier454166 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306

Subunit structure

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity. HAMAP-Rule MF_00306

Subcellular location

Cytoplasm. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity. HAMAP-Rule MF_00306

Domain

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily. HAMAP-Rule MF_00306

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding107 – 1148GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding190 – 1945GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding248 – 2514GTP By similarity HAMAP-Rule MF_00306

Sequences

Sequence LengthMass (Da)Tools
B5F291 [UniParc].

Last modified October 14, 2008. Version 1.
Checksum: CF0F70616A2111BD

FASTA45349,799
        10         20         30         40         50         60 
MFDNLTDRLS RTLRNISGRG RLTEDNVKET LREVRMALLE ADVALPVVRE FINRVKEKAV 

        70         80         90        100        110        120 
GHEVNKSLTP GQEFVKIVRS ELVAAMGEEN QTLNLAAQPP AVVLMAGLQG AGKTTSVGKL 

       130        140        150        160        170        180 
GKFLREKHKK KVLVVSADVY RPAAIKQLET LAEQVGVDFF PSDVGQKPVD IVNAALKEAK 

       190        200        210        220        230        240 
LKFYDVLLVD TAGRLHVDEA MMDEIKQVHA SIKPVETLFV VDAMTGQDAA NTAKAFNEAL 

       250        260        270        280        290        300 
PLTGVVLTKV DGDARGGAAL SIRHITGKPI KFLGVGEKTD ALEPFHPDRI ASRILGMGDV 

       310        320        330        340        350        360 
LSLIEDIESK VDRAQAEKLA TKLKKGDGFD LNDFLEQLKQ MKNMGGMASL MGKLPGMGQI 

       370        380        390        400        410        420 
PDNVKSQMDD KVLVRMEAII NSMTLKERAK PEIIKGSRKR RIAQGCGMQV QDVNRLLKQF 

       430        440        450 
DDMQRMMKKM KKGGMAKMMR SMKGMMPPGF PGR

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References

[1]"Complete genome of Salmonella agona strain SL483."
Ravel J., Fricke W.F., White D., McDermott P., Mammel M., Rosovitz M., Leclerc J., Cebula T., Sebastian Y.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SL483.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001138 Genomic DNA. Translation: ACH48818.1.
RefSeqYP_002147614.1. NC_011149.1.

3D structure databases

ProteinModelPortalB5F291.
SMRB5F291. Positions 2-431.
ModBaseSearch...

Protein-protein interaction databases

STRING454166.SeAg_B2823.
B5F291.

Proteomic databases

PRIDEB5F291.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACH48818; ACH48818; SeAg_B2823.
GeneID6797022.
KEGGsea:SeAg_B2823.
PATRIC18483922. VBISalEnt65316_2756.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0541.
HOGENOMHOG000036164.
KOK03106.
OMAGQQVVKI.
ProtClustDBPRK10867.

Enzyme and pathway databases

BioCycSENT454166:GHBA-380-MONOMER.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47446. Signal_recog_particle_SRP54_M. 1 hit.
SSF47364. SRP54. 1 hit.
TIGRFAMsTIGR00959. ffh. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB5F291_SALA4
AccessionPrimary (citable) accession number: B5F291
Entry history
Integrated into UniProtKB/TrEMBL: October 14, 2008
Last sequence update: October 14, 2008
Last modified: May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info