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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Salmonella agona (strain SL483)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi81Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:SeAg_B2394
OrganismiSalmonella agona (strain SL483)
Taxonomic identifieri454166 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000008819 Componenti: Chromosome

Subcellular locationi

B5EYU5:
  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_100018636932 – 828Periplasmic nitrate reductaseAdd BLAST797

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB5EYU5.
SMRiB5EYU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 954Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000031441.
KOiK02567.
OMAiTTDEVWP.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B5EYU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSRRSFMK ANAVAAAAAA AGLSVPGVAR AVVGQQEAIK WDKAPCRFCG
60 70 80 90 100
TGCGVLVGTQ QGRVVACQGD PDAPVNRGLN CIKGYFLPKI MYGKDRLTQP
110 120 130 140 150
MLRMKDGSYH KDGEFTPVSW EQAFDVMEEK FKTSLKEKGP EAIGMFGSGQ
160 170 180 190 200
WTIWEGYAAA KLFKAGFRSN NIDPNARHCM ASAVVGFMRT FGMDEPMGCY
210 220 230 240 250
DDIEQADAFV LWGSNMAEMH PILWSRITNR RLSDPNVKVA VLSTFQHRSF
260 270 280 290 300
ELADNGIVFT PQSDLVILNY IANYIIQNNA VNQDFFTKHV NLRKGATDIG
310 320 330 340 350
YGLRPTHPLE KAAKNPGSDA SEPMSFDEYK AFVAEYTLDK TAEMTGVPKD
360 370 380 390 400
QLEQLAQLYA DPNKRVISYW TMGFNQHTRG VWANNLVYNL HLLTGKISQP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNEKHRD ICEKHWQIPA
460 470 480 490 500
GTIPAKVGLH AVAQDRALKD GKLNVYWVMC NNNMQAGPNI NEDRMPGWRD
510 520 530 540 550
PRNFIIVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWRQQIK
560 570 580 590 600
APGEAKSDLW QLVQFSRRFK TEEVWPEALL AQKPELRGKT LYDVLFATPA
610 620 630 640 650
VSKFPLSELK EDQLNDESRE LGFYLQKGLF EEYAWFGRGH GHDLAPFDDY
660 670 680 690 700
HNARGLRWPV VEGKETQWRY SEGNDPYVKA GEGYKFYGKP DGKAVIFALP
710 720 730 740 750
FEPAAESPDN EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPEAVVFIHP
760 770 780 790 800
LDAKARDLRR GDKVKVSSRR GEVISIVETR GRNRPPQGLV YMPFFDAAQL
810 820
VNNLTLDATD PLSKETDFKK CAVKLAKV
Length:828
Mass (Da):92,874
Last modified:October 14, 2008 - v1
Checksum:i0CF99BC8B2509A85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001138 Genomic DNA. Translation: ACH49382.1.
RefSeqiWP_000778097.1. NC_011149.1.

Genome annotation databases

EnsemblBacteriaiACH49382; ACH49382; SeAg_B2394.
KEGGisea:SeAg_B2394.

Similar proteinsi

Entry informationi

Entry nameiNAPA_SALA4
AccessioniPrimary (citable) accession number: B5EYU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 14, 2008
Last modified: October 25, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families