B5ES06 (PANC_ACIF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Lferr_1446

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Pantothenate synthetase HAMAP MF_00158		PRO_1000118139
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity
	Nucleotide binding	147 – 150	4	ATP By similarity
	Nucleotide binding	184 – 187	4	ATP By similarity
Sites
	Active site	34	1	Proton donor By similarity
	Binding site	58	1	Beta-alanine By similarity
	Binding site	58	1	Pantoate By similarity
	Binding site	153	1	Pantoate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5ES06 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: F2B5588544C6720E	FASTA	278	30,789
	10 20 30 40 50 60 MAIFKDIAGL RQWRQSLHGT LALVPTMGNL HEGHLALVRL AANRAEHVLV SIYVNPLQFG 70 80 90 100 110 120 PGEDFANYPR TLDQDLQRLH EAGCQTVFTP DDGLMYPRGR QDISIVMPPR SLSKVLCGAS 130 140 150 160 170 180 RPGHFAGVCT VLSKLLHMVA PEILILGEKD YQQLRIVQQM VADLNLNVQV LPGPLQREAD 190 200 210 220 230 240 GLAYSSRNIY LNLAERQVAP LLAETLFDLA RRSTNDAAVS DLAATGWERL ERAGFLPEYL 250 260 270 ELRDAQTLQS LALPQPGARW FAAARLGQIR LIDNVIIS

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 53993.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH83677.1.
RefSeq	YP_002219884.1. NC_011206.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5ES06.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6877420.
GenomeReviews	Gene locus Lferr_1446 in contig CP001132_GR.
KEGG	afe:Lferr_1446.
PATRIC	20660482. VBIAciFer6930_1445.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG428839.
OMA	EDFGSYP.
ProtClustDB	PRK00380.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_ACIF5

Accession

Primary (citable) accession number: B5ES06

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program