B5EQG3 (PYRB_ACIF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	Lferr_2666

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_1000088732

Sequences

	Sequence		Length	Mass (Da)
	B5EQG3 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 2E7BA1B6E5ED5323	FASTA	327	35,321
	10 20 30 40 50 60 MSATLRFGEG NLQYDAQGRL RHLLSTEGLR ERELLQILDT AESFLSIANR SVKKTPTLRG 70 80 90 100 110 120 RTIANLFFEN STRTRSTFEL AAKRLSADVL NIAVSTSSAS KGESLTDTID NLMAMQVDGF 130 140 150 160 170 180 VIRHPEAGAA HLVARHLGDS ALVVNAGDGQ HAHPTQALLD VFTIRRLGGP IEDRVVAIVG 190 200 210 220 230 240 DVFHSRVARS QIHALSVLGC PEIRVIGPRT LVPEELSALG VHVYHDLQAG LRGVDVICAL 250 260 270 280 290 300 RLQRERMESH RLPSLDEFHR RFGLTPERLQ WAEPGALVLH PGPMNRGVEI ASEVADGAQA 310 320 VILQQVAHGL AVRMAVLAIL AGAAGGA

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 53993.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH84860.1.
RefSeq	YP_002221067.1. NC_011206.1.
3D structure databases
ProteinModelPortal	B5EQG3.
ModBase	Search...
Protein-protein interaction databases
STRING	B5EQG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6878665.
GenomeReviews	Gene locus Lferr_2666 in contig CP001132_GR.
KEGG	afe:Lferr_2666.
PATRIC	20662926. VBIAciFer6930_2644.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	LQRERMS.
ProtClustDB	PRK00856.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_ACIF5

Accession

Primary (citable) accession number: B5EQG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program