B5EQ26 (PYRE_ACIF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	Lferr_1029

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000138756
Regions
	Region	117 – 125	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	121	1	Orotate By similarity
	Binding site	149	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5EQ26 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: D617C3A5AA5579AA	FASTA	195	20,221
	10 20 30 40 50 60 MTADEVVALY KEDGALLHGH FLLSSGLHSD TYLQSARVLQ YPGHAARLCA AMVAGIPDDL 70 80 90 100 110 120 RRRISCVVGP AMGAVLVSYE CGRALGVRSL FTEREAGQMV LRRGFVLAPG EGVLVVEDIT 130 140 150 160 170 180 TTGGSTRECM AAVTAAGGQV LAASAIIDRS SGFSDFDGAP FFPLLKLPVL TWEAAACPLC 190 AAGGTPVKPG SRGLS

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 53993.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH83271.1.
RefSeq	YP_002219478.1. NC_011206.1.
3D structure databases
ProteinModelPortal	B5EQ26.
ModBase	Search...
Protein-protein interaction databases
STRING	B5EQ26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6876998.
GenomeReviews	Gene locus Lferr_1029 in contig CP001132_GR.
KEGG	afe:Lferr_1029.
PATRIC	20659650. VBIAciFer6930_1033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	CLSK2407751.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_ACIF5

Accession

Primary (citable) accession number: B5EQ26

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program