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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211OrotateUniRule annotation
Binding sitei149 – 1491OrotateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciAFER380394:GHE0-1049-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:Lferr_1029
OrganismiAcidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993))
Taxonomic identifieri380394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAcidithiobacilliaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
Proteomesi
  • UP000001856 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Orotate phosphoribosyltransferasePRO_1000138756Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi380394.Lferr_1029.

Structurei

3D structure databases

ProteinModelPortaliB5EQ26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 12595-phosphoribose 1-diphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107VCP. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000059806.
KOiK00762.
OMAiGHFRLTS.
OrthoDBiPOG091H034Q.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR006273. Orotate_PRibTrfase_bac.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01367. pyrE_Therm. 1 hit.

Sequencei

Sequence statusi: Complete.

B5EQ26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTADEVVALY KEDGALLHGH FLLSSGLHSD TYLQSARVLQ YPGHAARLCA
60 70 80 90 100
AMVAGIPDDL RRRISCVVGP AMGAVLVSYE CGRALGVRSL FTEREAGQMV
110 120 130 140 150
LRRGFVLAPG EGVLVVEDIT TTGGSTRECM AAVTAAGGQV LAASAIIDRS
160 170 180 190
SGFSDFDGAP FFPLLKLPVL TWEAAACPLC AAGGTPVKPG SRGLS
Length:195
Mass (Da):20,221
Last modified:October 14, 2008 - v1
Checksum:iD617C3A5AA5579AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001132 Genomic DNA. Translation: ACH83271.1.
RefSeqiWP_012536428.1. NC_011206.1.

Genome annotation databases

EnsemblBacteriaiACH83271; ACH83271; Lferr_1029.
KEGGiafe:Lferr_1029.
PATRICi20659650. VBIAciFer6930_1033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001132 Genomic DNA. Translation: ACH83271.1.
RefSeqiWP_012536428.1. NC_011206.1.

3D structure databases

ProteinModelPortaliB5EQ26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380394.Lferr_1029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACH83271; ACH83271; Lferr_1029.
KEGGiafe:Lferr_1029.
PATRICi20659650. VBIAciFer6930_1033.

Phylogenomic databases

eggNOGiENOG4107VCP. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000059806.
KOiK00762.
OMAiGHFRLTS.
OrthoDBiPOG091H034Q.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciAFER380394:GHE0-1049-MONOMER.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR006273. Orotate_PRibTrfase_bac.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01367. pyrE_Therm. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_ACIF5
AccessioniPrimary (citable) accession number: B5EQ26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 14, 2008
Last modified: September 7, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.