B5EPM6 (KDSA_ACIF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	Lferr_0974

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_1000116874

Sequences

	Sequence		Length	Mass (Da)
	B5EPM6 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: A1179842190CD5FB	FASTA	281	30,604
	10 20 30 40 50 60 MRLCGFEAGL QHPFFLMAGP CAIESESLAL RTAEDLRDIC ARLGIPFIYK SSYDKANRSS 70 80 90 100 110 120 GQSFRGPGMD EGLRILEKVR REVGVPVVTD VHEKEDVSAV AEVVDVLQTP AFLCRQTDFI 130 140 150 160 170 180 QAVAAAGKPV NIKKGQFLAP WDMLHVASKA KATGNEQIMV CERGASFGYN NLVSDMRSLA 190 200 210 220 230 240 VMRQTGCPVV FDATHSVQLP GGQGDRSGGQ REFIPVLARA AVAAGVSGLF METHPNPADA 250 260 270 280 LSDGPNAWPL GRMEDLLRIL QHIDHVVKNQ DFPENYPEEL V

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 53993.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH83220.1.
RefSeq	YP_002219427.1. NC_011206.1.
3D structure databases
ProteinModelPortal	B5EPM6.
ModBase	Search...
Protein-protein interaction databases
STRING	B5EPM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6876940.
GenomeReviews	Gene locus Lferr_0974 in contig CP001132_GR.
KEGG	afe:Lferr_0974.
PATRIC	20659544. VBIAciFer6930_0983.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG575990.
OMA	ESRDHAF.
ProtClustDB	PRK05198.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_ACIF5

Accession

Primary (citable) accession number: B5EPM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program