Reviewed, UniProtKB/Swiss-Prot B5ENA8 (SYM_ACIF5)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Lferr_2296

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_1000199269
Regions
	Domain	577 – 678	102	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	328 – 332	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	331	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5ENA8-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 3EFA477B7318EF42	FASTA	678	75,014
	10 20 30 40 50 60 MKRRILITSA LPYANGPIHL GHLVEYTQTD IWARYQRLRG HDCVYVCADD AHGTPIMLRA 70 80 90 100 110 120 QSEGITPEEL ITRMHGDHLR DFTGFGIRFD LYHSTHSPEN FEISQSIYRA LRAADYINVR 130 140 150 160 170 180 EIEQAYDPVA GIFLPDRFIR GTCPRCGAAD QYGDSCEVCG ATYSPTDLIN PVSAVSGAVP 190 200 210 220 230 240 ERRNSEHYFF QLGDFSDFLR RWIHSGTLQE EVAHKLDEWF SIGLSDWDIS RDAPYFGIPI 250 260 270 280 290 300 PDAPGKFFYV WLDALPGYMA ATQHWCAAHG RNLADYWGPD SAAEIYHFIG KDIIYFHGLF 310 320 330 340 350 360 WPAMLKGSGH RLPTGIFAHG HLTVNGAKMS KSRGTSITAR QYLQHLNPEF LRYYIATKLN 370 380 390 400 410 420 SHVEDIDLNL EDFLLRGNGD LVGKVVNLAS RAAGFIHRSF AGRLAASLGK DQAFYDGLLQ 430 440 450 460 470 480 TQEAIGEAYA GREYGKAMRD IMALADQINA YVDQNAPWTL AKDPAQHESL HRVVTVTLNG 490 500 510 520 530 540 FRVLITLLSP VLPELSRKAL EFLQCELDWA GLSKPLLDHQ ILPYSHLLQR MEKTQVDALI 550 560 570 580 590 600 QNPAESPATA PGTAAAPVPT PVPAEAREEN PFIGIDDFSK VDLRIARIVA ATNVDGADKL 610 620 630 640 650 660 LHLTLDIGEG TRSVFAGIKS AYDPASLVGR LTVMVANLAP RKMRFGLSEG MVLAASGPEG 670 GPFLLSPDSG AQPGMRVK

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001132 Genomic DNA. Translation: ACH84497.1.
RefSeq	YP_002220704.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6878290.
GenomeReviews	Gene locus Lferr_2296 in contig CP001132_GR.
KEGG	afe:Lferr_2296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SAYQPEQ.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ACIF5

Accession

Primary (citable) accession number: B5ENA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)