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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per monomer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi146Zinc 11
Metal bindingi149Zinc 11
Metal bindingi163Zinc 21
Metal bindingi166Zinc 21
Metal bindingi185Zinc 21
Metal bindingi188Zinc 21
Metal bindingi199Zinc 11
Metal bindingi202Zinc 11

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 211CR-typeAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJ
Gene namesi
Name:dnaJ
Ordered Locus Names:Lferr_2290
OrganismiAcidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993))
Taxonomic identifieri380394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAcidithiobacilliaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
Proteomesi
  • UP000001856 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001376541 – 375Chaperone protein DnaJAdd BLAST375

Proteomic databases

PRIDEiB5ENA2.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi380394.Lferr_2290.

Structurei

3D structure databases

ProteinModelPortaliB5ENA2.
SMRiB5ENA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 70JAdd BLAST66
Repeati146 – 153CXXCXGXG motif8
Repeati163 – 170CXXCXGXG motif8
Repeati185 – 192CXXCXGXG motif8
Repeati199 – 206CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 116Gly-richAdd BLAST40

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.

Sequence similaritiesi

Belongs to the DnaJ family.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 211CR-typeAdd BLAST79

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.
OrthoDBiPOG091H00PT.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR036869. DnaJ_dom_sf.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

B5ENA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATRDYYEVL EISRTADDGE IKKSYRRLAM RYHPDRNPDD ASAEERFKEI
60 70 80 90 100
SAAYEVLSDP QKRQAYDRFG HAGVNGGGGG PGAGFGGGGG GFGDVFSDLF
110 120 130 140 150
EQAFGGGFRG QDSGRGADLR YELELTLEEA ALGKEVTIQI PSSATCEVCR
160 170 180 190 200
GSGAKPGSAV EDCATCGGRG QVRMVQGFFS VTRPCPQCNG SGKVIKEPCT
210 220 230 240 250
NCHGHGRVRR NRDLQVKVPA GVDTGDRIRL NGEGEAGERG GPAGDLYIQV
260 270 280 290 300
RVLPHALFER DGDDLHCAVP VQFTTMAMGG ELEVPTLTGR AKVQIAPGTQ
310 320 330 340 350
SGAVFRLRGK GIKGVRSKLN GDLHCQLQVE VPVHLSARQK ELLEEFAREG
360 370
GDNIQHPQQE SWWNKAKDFF DRMGL
Length:375
Mass (Da):40,516
Last modified:October 14, 2008 - v1
Checksum:iE1D658AC7428AACC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001132 Genomic DNA. Translation: ACH84491.1.
RefSeqiWP_012537323.1. NC_011206.1.

Genome annotation databases

EnsemblBacteriaiACH84491; ACH84491; Lferr_2290.
KEGGiafe:Lferr_2290.

Similar proteinsi

Entry informationi

Entry nameiDNAJ_ACIF5
AccessioniPrimary (citable) accession number: B5ENA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 14, 2008
Last modified: October 25, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families