B5EN71 (NUOA_ACIF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A
NDH-1 subunit A
NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	Lferr_2257

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_5000395043
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	B5EN71 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 54EEB48D8599169F	FASTA	118	13,500
	10 20 30 40 50 60 MLNHYLPVLI FLLVALVVGV APLLMGSSLG PHRPDSEKLS PYECGFEAFE DARMKFDVRY 70 80 90 100 110 YLVAILFILF DLEIAFLFPW AVVFDQIGMT GFLAMMLFLA ILVVGFIYEW KKGALEWE

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 53993.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH84460.1.
RefSeq	YP_002220667.1. NC_011206.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5EN71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6878250.
GenomeReviews	Gene locus Lferr_2257 in contig CP001132_GR.
KEGG	afe:Lferr_2257.
PATRIC	20662118. VBIAciFer6930_2246.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG605540.
OMA	IGFAYEW.
ProtClustDB	CLSK2408394.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_ACIF5

Accession

Primary (citable) accession number: B5EN71

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program