Reviewed, UniProtKB/Swiss-Prot B5ELU7 (METE_ACIF5)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	Lferr_0465

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 784	784	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097811
Sites
	Metal binding	670	1	Zinc By similarity
	Metal binding	672	1	Zinc By similarity
	Metal binding	755	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5ELU7-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: FD779F59D595B16C	FASTA	784	87,928
	10 20 30 40 50 60 MTSVHSLGFP RIGHKRELKK ALESFWSREI DEQELQSRAA QLRDRHWRIQ QTCGMDLVPV 70 80 90 100 110 120 GDFSLYDHML DMSCTLGAIP PRYGFAGGQV GLDTFFAMAR GSATQPAMEM TKWFDTNYHF 130 140 150 160 170 180 IVPEFHEGMD FRLSSERLFD QVKEVQALGL KAKPVLVGPI TYLWLGKEKD LNAEAHHDAQ 190 200 210 220 230 240 HHHDDSACHG HGAPIGAACF DRLTLLPKVL PVYAEILARL AEMGVEWVQI DEPALALDLP 250 260 270 280 290 300 QEWVEALESA YQTLRRDKTP KVLLATYFDS VADHAKALKA LPVAGVHLDL RRAPQQLNSF 310 320 330 340 350 360 LSDYPADKVL SLGVVDGRNV WRADLDAALE LLQPAHKQLG DRLWVAPSCS LLHTPVDLEQ 370 380 390 400 410 420 ETELDAELKS WLSFSVQKLD EVAIIGRALK EGVESVAQEL AAARAAVASR KSSPRIHNPA 430 440 450 460 470 480 VAQRLEGLGQ DDGRRKSPFP IREAAQRARF KLPAFPTTSI GSFPQTPEIR KARLQNRKGE 490 500 510 520 530 540 LSNADYQKAM EAEIALVVKE QERLGIDVPV HGEPERNDMV EYFGEQLAGF AFTRHGWVQS 550 560 570 580 590 600 YGSRYVKPPL IFGDVSRPTP MTVAWSKYAQ SLTQRPMKGM LTGPVTILQW SFVRDDQPRE 610 620 630 640 650 660 RTALQIALAI RDEVRDLIDA GIGIIQIDEP AYREGLPLKR KDWGHYLEWA SRAFRISAQI 670 680 690 700 710 720 APDDVQIHTH MCYSEFNDIL PAIAAMDADV ITIETSRSQM ELLDAFATFN YPNEIGPGVY 730 740 750 760 770 780 DIHSPRVPSV EEMVGLMEKA VKVVPAERLW INPDCGLKTR KWAEVTPALE NMVEAARQVR ARHG

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH82719.1.
RefSeq	YP_002218926.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6876417.
GenomeReviews	Gene locus Lferr_0465 in contig CP001132_GR.
KEGG	afe:Lferr_0465.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	SFVRNDI.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_ACIF5

Accession

Primary (citable) accession number: B5ELU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	February 9, 2010
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)