Reviewed, UniProtKB/Swiss-Prot B5ELC2 (MURC_ACIF5)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Lferr_0384

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000091072
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B5ELC2-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 6B3097FEF9CFBA03	FASTA	464	49,171
	10 20 30 40 50 60 MRNWVRQIHM VGIGGSGMRG IAEVLLNLGY AVSGSDLRPG NSTLRLTDLG ARIFSGHEAA 70 80 90 100 110 120 NVRGADVVVI SSAIPESNPE VQAARELRIP VIRRAEMLAE LMRFKQGIAI AGTHGKTTTT 130 140 150 160 170 180 SLVASILGAG GLDPTFVIGG RLKSAGTHAA LGSGEYLVAE ADESDASFLY LSPVMAVVTN 190 200 210 220 230 240 IDADHMETYG GSLDNLRGAF LQFLQRLPFY GLAVLCTDEA VVAGLIPELR TPVLRYGFGA 250 260 270 280 290 300 DADLQARDVT VQGIGSRFAV WRRVDSDYVH WLDVELGIPG RHNVLNALAA IGIASKVGIP 310 320 330 340 350 360 ESTIATALAD FRGVGRRFEL VGTFDGITVV DDYGHHPREI AATIAAARSV WPERPLVVAF 370 380 390 400 410 420 QPHRYSRTQA LFADFVSSLA AADRVILTDI YSAGEKALPG VTGRALAEAM AAQGMSVRFL 430 440 450 460 PDLLTAPQQI RAELPAGAVL LTLGAGSIAS LAAQWPQVFG EDPS

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001132 Genomic DNA. Translation: ACH82638.1.
RefSeq	YP_002218845.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6876335.
GenomeReviews	Gene locus Lferr_0384 in contig CP001132_GR.
KEGG	afe:Lferr_0384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACIF5

Accession

Primary (citable) accession number: B5ELC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)