Reviewed, UniProtKB/Swiss-Prot B5EL87 (SYR_ACIF5)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Lferr_0349

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 579	579	Arginyl-tRNA synthetase HAMAP MF_00123		PRO_1000095329
Regions
	Motif	127 – 137	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B5EL87-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 733F5D6CCB2118DC	FASTA	579	64,520
	10 20 30 40 50 60 MKAFVSQALQ GALQQLHAQG RIPGIPATLE LDRPKQVEHG HLASNVALLL AKAVGRKPRD 70 80 90 100 110 120 IADDIVAALP ASDWIARTEI AGPGFINFFL QPAAFHAVIH RVRTEKEHFG ANRNGAGQRL 130 140 150 160 170 180 QMEFVSANPT GPLHVGHGRG AAYGASLANI LRFNGFDIFC EYYVNDAGRQ MDILAASVYL 190 200 210 220 230 240 RYLEADKALP WPFPENGYRG DYVREIAAHL REQVGDRLRH AAVGLPNLPQ MSDGDIAIDT 250 260 270 280 290 300 LIAHLKQSLG EDYRTLHSAG LDEILADIRD DLEGFGVHYE RWYSEGSLMD TGAVDSAVAA 310 320 330 340 350 360 LEKAGHCYTQ EGALWFRATA FDDDKDRVLR RDNGAYTYFA SDVAYHAEKF ARGFTHVIDM 370 380 390 400 410 420 WGADHHGYVP RVKAALRALG LDDQQLEVVL VQFAILYRGT EKISMSTRAG EFVTLRELRE 430 440 450 460 470 480 EVGNDAARFF YVLRRADQHL DFDLELAKKH SEENPVFYIQ YAHARVYSLL RQSVEKGLSL 490 500 510 520 530 540 PPADGVGLEI LQESREIALA DALWRFPEVV ATAARDREPH QIAFYLRELA AAFHTYYNST 550 560 570 RILVEETPLR HARLTLCLAV AQSIANGLRL LGVSAPEQM

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001132 Genomic DNA. Translation: ACH82603.1.
RefSeq	YP_002218810.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6876300.
GenomeReviews	Gene locus Lferr_0349 in contig CP001132_GR.
KEGG	afe:Lferr_0349.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADHHGYV.
Family and domain databases
HAMAP	MF_00123. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_ACIF5

Accession

Primary (citable) accession number: B5EL87

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	November 3, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)