Reviewed, UniProtKB/Swiss-Prot B5EL46 (PYRD_ACIF5)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Lferr_1911

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000195055
Sites
	Active site	181	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5EL46-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 2BA24DF5C2099453	FASTA	363	38,448
	10 20 30 40 50 60 MSYGLLRPLL FTLDPERAHT LSIAALEALG HMPRGLARVA RRYTAHDPRL AQDFWGLHFA 70 80 90 100 110 120 NPVGLAAGYD KDARATAALP ALGFGFIEIG TVTPRPQPGN PRPRVFRYPA QQAVINRMGF 130 140 150 160 170 180 PGEGAAAVAR RLAALPGHPV PIGINLGKNK DTPLERAQDD YVAALELLFH YGDYLCINVS 190 200 210 220 230 240 SPNTPGLRLL QGEEALRGLL KAVAAANQRL ALQHQRPPLP LLLKIAPDLD NDDLNAIGSL 250 260 270 280 290 300 ALGTAPLVNG FIATNTTIER PASQPGLSES GGLSGAPLLQ QSNAVIAQLY RATQGQVPII 310 320 330 340 350 360 GVGGILSAAD AYAKILAGAS LVQVYSGLIF RGPGLVREIL EELPGLWLKD GYPDLAHARG STA

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001132 Genomic DNA. Translation: ACH84132.1.
RefSeq	YP_002220339.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6877896.
GenomeReviews	Gene locus Lferr_1911 in contig CP001132_GR.
KEGG	afe:Lferr_1911.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	AALNRMG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ACIF5

Accession

Primary (citable) accession number: B5EL46

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 14, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)