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B5EL11

- SYE2_ACIF5

UniProt

B5EL11 - SYE2_ACIF5

Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (14 Oct 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131ZincUniRule annotation
    Metal bindingi115 – 1151ZincUniRule annotation
    Metal bindingi140 – 1401ZincUniRule annotation
    Metal bindingi142 – 1421ZincUniRule annotation
    Binding sitei260 – 2601ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAFER380394:GHE0-1912-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetase 2UniRule annotation
    Short name:
    GluRS 2UniRule annotation
    Gene namesi
    Name:gltX2UniRule annotation
    Ordered Locus Names:Lferr_1876
    OrganismiAcidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993))
    Taxonomic identifieri380394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
    ProteomesiUP000001856: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482Glutamate--tRNA ligase 2PRO_0000367598Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi380394.Lferr_1876.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi16 – 2611"HIGH" regionAdd
    BLAST
    Motifi257 – 2615"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252721.
    KOiK01885.
    OMAiKEDIAKP.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5EL11-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQYYTTMNL KSRFAPSPTG YLHLGNARTA LFAWLAARGA GGTFILRLED    50
    TDQQRSPEIY ERALLEDLRW FGIDWDEGPD RGGDHGPYRQ MERLAVYGRY 100
    YAHLQSSGQA YACYCSADDL AAERAVQRSA GKAPRYGGRC RHLDAAARAE 150
    REAAGLLPTL RFRVPDQGTL TVPDLVWGER HYALADLGDF VIRRSDGSPA 200
    FFFANAVDDA LMEVNLVLRG EDHLTNTPRQ ILILQALGLP VPAYGHLPLL 250
    LGADGQPLSK RYGAASLRDL RKDGYLAAAL RNYLARLGHH YSATGFLDSR 300
    ALAQGFALNQ ISRAPSHFDM VQLQHWQHEA VQSLDDAAVW NWLAPLLRGK 350
    VPMGLEMPFV AAVRANILLP GDALDWAERC FGAPALAADA VEAITGVSPE 400
    FWSVAQATLQ ASGGDYRRWT KALQQASGRR GKALFMPLRA ALTGLTHGPE 450
    LAALLPLIGE ERALARLHAA ARRPSTPIET TL 482
    Length:482
    Mass (Da):52,887
    Last modified:October 14, 2008 - v1
    Checksum:iEE70AFD13CE7C015
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001132 Genomic DNA. Translation: ACH84097.1.
    RefSeqiYP_002220304.1. NC_011206.1.

    Genome annotation databases

    EnsemblBacteriaiACH84097; ACH84097; Lferr_1876.
    GeneIDi6877861.
    KEGGiafe:Lferr_1876.
    PATRICi20661358. VBIAciFer6930_1874.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001132 Genomic DNA. Translation: ACH84097.1 .
    RefSeqi YP_002220304.1. NC_011206.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 380394.Lferr_1876.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACH84097 ; ACH84097 ; Lferr_1876 .
    GeneIDi 6877861.
    KEGGi afe:Lferr_1876.
    PATRICi 20661358. VBIAciFer6930_1874.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252721.
    KOi K01885.
    OMAi KEDIAKP.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci AFER380394:GHE0-1912-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P.
      Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 53993.

    Entry informationi

    Entry nameiSYE2_ACIF5
    AccessioniPrimary (citable) accession number: B5EL11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3