B5EKZ2 (AMPA_ACIF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Lferr_1857

Organism

Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)) [Complete proteome] [HAMAP]

Taxonomic identifier

380394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000098300
Sites
	Active site	281	1	Potential
	Active site	355	1	Potential
	Metal binding	269	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 1 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	292	1	Manganese 2 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5EKZ2 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 7E950A380AC66BF1	FASTA	500	52,844
	10 20 30 40 50 60 MEIAVQCQNP TTDNSDCVVV GIYEGGILSP AATLVDLASG GALRALLDTG DFTGDCGDTQ 70 80 90 100 110 120 LLYQVPGMAA ARVLVLGLGS HGKVKDSQFR KAALAAARAL QGARVGRASL HLLDTPVIRR 130 140 150 160 170 180 SAPACAKILV QAVADAEYHF DRHKKPADAP QRPISELQLS ISENDTAALA ELQGAVAEAQ 190 200 210 220 230 240 ATARAVAWTR DMANEPGNIC TPTWLAEQAE AMAGRLGIKS TILGPDAMEA LGMHLLLGVA 250 260 270 280 290 300 HGSRQPPRLI ILEYRGGAEN QAPIVLVGKG ITFDAGGISL KPADKMDEMK YDMCGGASAL 310 320 330 340 350 360 AAIQAAAELQ LPLNIVTVVP ASENLPDGQA TKPGDIHRSM NGLSVEVVNT DAEGRLILAD 370 380 390 400 410 420 TLTYVERFEP DVVIDMATLT GACIIALGHQ TAAVMGNHEG LVHDLIAAGK ESMDRVWELP 430 440 450 460 470 480 LFEEYQEQLK SPVADLSNVG GRPAGTITAA CFLSRFTENY RWAHLDIAGV AWKSGEHKGA 490 500 TGRPVPLLVE YLLRRARQVA

References

[1]

"Complete sequence of Acidithiobacillus ferrooxidans ATCC 53993." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Borole A.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 53993.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001132 Genomic DNA. Translation: ACH84078.1.
RefSeq	YP_002220285.1. NC_011206.1.
3D structure databases
ProteinModelPortal	B5EKZ2.
ModBase	Search...
Protein-protein interaction databases
STRING	B5EKZ2.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6877841.
GenomeReviews	Gene locus Lferr_1857 in contig CP001132_GR.
KEGG	afe:Lferr_1857.
PATRIC	20661314. VBIAciFer6930_1853.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	KYDWAHL.
ProtClustDB	CLSK2408208.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ACIF5

Accession

Primary (citable) accession number: B5EKZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program